The antigen that we are trying to detect in pathogens using monoclonal antibody or polyclonal antibody studies, are these not detected when they the organism to be studied is dead?
In addition to this, the protein (antigen), is it always present in the pathogen or is it heavily biologically secreted in pathogens when the pathogen are about to cause disease or produce toxin?
Does the antigen production differ in a pathogen at different stages or is it consistently same?
Please do a literature search on expression pattern of the protein in your pathogen. Furthermore, if the antibody is specific for the antigen of your pathogen. Also if any studies have used a particular Ab please try them. Generally monoclonal are more specific than polyclonal.
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