What could be source of contamination for seeing positive amplification in negative control?

Yesterday, I ran a gradient endpoint PCR with some primers and saw positive amplification for positive and negative for negative amplification. Running the same assay today, show positive...

13 January 2021 5,201 1 View

Has anybody found better anticancer effect on patient derived primary cancer cells than cell line?

I am testing compounds on the patient's primary cancer cells as well as a cell line. Generally, I see better activity in cell lines compared to primary cells. Surprisingly I am seeing the...

18 March 2020 467 3 View

Catalyst and electrode for fuel cell application?

Hello, could anybody help me to get idea about which kind of ionomers, surfactants, additives and solvents except Nafion, iso-propanol, n-propanol, hexanol, methanol, ethanol, water, ePTFE,...

11 November 2018 8,886 7 View

Regarding K Means Clustering Technique?

Hello Experts, Can we use K Means Clustering for predicting Software Maintainability at design level?

30 March 2016 7,230 4 View

Regarding Software Maintainability ?

Sir, I have taken 22 different UML Class diagram( samples) of 2 different variables and first one contains 4 independent variables and second one contains 7 independent variables.In this situation...

10 February 2016 1,372 2 View

How to find out understandability, analysability and modifiability?

08 February 2016 2,605 1 View

Does anyone have experience with Isolation of sesquiterpene?

Which solvent systems hould be used to make the plant extract. I am not having facility of reverse phase so by using silica how can I isolate the sesquiterpenes

06 July 2013 3,822 1 View

How to isolate terpenes and sesquiterpenes from methnolic extract

How to isolate terpenes and sesquiterpenes from methnolic extract

05 December 2012 1,528 11 View

What is the usual concentration of Diaporthe fungus using DNeasy mini kit protocol?

Hello Everyone, I am using DNeasy mini protocol to extract DNA from at least 7 days old culture in PDA. When I tried it, several times I am getting less than 10 ng/micro liter of DNA. It is...

01 January 1970 2,960 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Is there any published or unpublished statistics on dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, US, UK or any other country?

Are there any published or unpublished statistics or papers on the dropout rate (Dose1 to Dose-2) for Covid-19 vaccination in India, USA, UK, or any other country where vaccination has started at...

28 February 2021 4,237 1 View