I am been using VEROTMPRSS2 cells and incubating cells with 200ul of virus dilution for one hour. Cell layers are coming off after 30 min incubation. What might be the best solution to keep cell layer intact. is it with rocking the plate?
200ul for a P6 well is the minimum ... you need to rock the plate not with a circular movement (the center of the well will not be weted) but with a front/back and right/left movement ... you can do this cross movment by hand (if you don(t have the right rocker) each 15 min. (in a humidified Co2 incubator)