Bhawana Ghimire

4 Questions 5 Answers 0 Followers

Questions related from Bhawana Ghimire

Antibody (monoclonal and polyclonal) based method of fungal pathogen identification can be done invitro or does it need host?

The antigen that we are trying to detect in pathogens using monoclonal antibody or polyclonal antibody studies, are these not detected when they the organism to be studied is dead? In addition...

16 February 2022 9,010 1 View

Does anyone have a protocol on identifying dormant and alive spores of fungi and oomycetes?

The spores that I harvested are either not mature or dormant. How can we differentiate between dormant and alive spores? Maybe looking at the movement in oomycetes. How do we sort them?

14 June 2021 7,335 5 View

What could be source of contamination for seeing positive amplification in negative control?

Yesterday, I ran a gradient endpoint PCR with some primers and saw positive amplification for positive and negative for negative amplification. Running the same assay today, show positive...

14 January 2021 5,393 1 View

What is the usual concentration of Diaporthe fungus using DNeasy mini kit protocol?

Hello Everyone, I am using DNeasy mini protocol to extract DNA from at least 7 days old culture in PDA. When I tried it, several times I am getting less than 10 ng/micro liter of DNA. It is...

01 January 1970 3,041 3 View