I collected samples at 30min,1 hrs,2 hrs,6hrs,,12hrs and 24 hrs. But still couldn't detect phosphorylation. If anyone has done it, could you please suggest me better stimulation and collection time.
In our hands, pIC activates pIRF7 not 3 (see PMID: 37212952). We can trigger pIRF3 by LPS stimulation (3 h and 100 ng/ml). Hope this is helpful!.
Katharina Kubatzky Thank you for your response. I will look at it.
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