DNA pol quantification?

Currently, I'm working on a DNA pol activity test, one of the variables is DNA pol concentration in the PCR/LAMP assay. For LAMP I found a clear protocol for the DNA pol in weights. However, for...

01 January 2021 9,869 3 View

Vaccine, Adjuvant, and Drug Delivery System?

In terms of vaccine solution, the vaccine (ex: protein sub-unit based) is contained inside nanoparticles (ex: PLGA). Does the adjuvant inside the nanoparticle as well or act as a solvent that...

03 October 2020 4,500 4 View

Thrombin Fuction in Plasmid/Vector?

What is the function of thrombin in a plasmid? Does it help for screening, selection, or as a biomarker?

09 September 2020 1,555 3 View

Does international survey based researches needs approval in each country ethics commitee?

We plan a contingent valuation of ecosystem services. The study will be based on surveys. We want to apply it in fifteen different countries. Is it necessary to approve the project in ethics...

25 June 2020 8,099 3 View

What is the current importance of the popular legislative initiative for strengthening democracy?

The objective in formulating the questioning is the search to identify what is the feeling that the researchers have about the interactions between the strengthening of democracy through the...

12 June 2020 4,085 2 View

Can I Combine Data and Tree Information into One Tree in Mesquite?

Is it possible to enter two different types of information into Mesquite? I have two sets of species data: the divergence times (in millions of years) i.e. how old each species is and the...

22 March 2020 6,304 3 View

Does anyone know of a practical energy efficiency model for wireless sensor networks (WSN) or software that uses attributes of that model?

I have already found some reference authors who model some parts of the system, for example, sensor, transceivers, link, propagation space, the network itself and, sometimes, putting it all...

11 November 2019 2,111 3 View

What is the minimum level of energy that allows a near body device to be long term self-sustainable?

My concern is about Energy Efficiency Model for WBANs. In a wide sense, I'm looking for ways to model the whole network in terms of power and energy. How much average power is required to keep...

11 November 2019 5,053 4 View

Why Bluetooth, or more recently BLE, is today's dominant technology for communications on sensors and IoT devices?

Is that correct? Could you give a list of pros and cons when compared to a plethora of options? For instance, IR-UWB is simpler (hardware mainly) than narrowband options, although it works with...

11 November 2019 4,370 3 View

RNA-sequencing- High RIN but low DV200 on Tapestation?

I've recently sent off E. coli RNA samples for RNA-seq. The company we have used for sequencing have replied and said all samples failed QC due to degradation. I am looking at the Tapestation...

20 May 2019 8,093 3 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

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02 March 2021 3,060 3 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View