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I upload a genome to check using Busco via galaxy server. Currently, it is 2 days and the result is not finished yet? Did I miss something or is there is a problem? Thank you in advanced
23 November 2022 5,767 2 View
I tried using Phaster.ca and PhiSpy for phage detection in the bacterial genome They showed a completely different result for regions and the virus identified. Do you have the same experiences...
15 November 2022 8,898 6 View
Is there any server or tools (bioconda, java, etc.) to exclusively annotate membrane protein only (similar to dbCAN for polysaccharides) from a bacterial genome? Thank you in advanced!
30 September 2022 9,208 3 View
I use MEGA for making phyllo tree. But for poster presentation reason the tree is too big. Can I use MEGA to make clustering of multiple species? Or do I need something like ARB? Do you have any...
04 April 2022 3,892 2 View
How to decrease an Anaerobic Chamber from 22 ppm to 0 ppm. A few things come to mind (have not tried) : 1. Decrease the water evaporation rate (Needed to remove H2S) 2. Increase the temperature to...
21 February 2022 5,014 3 View
I tried several times making a Chitosan-Alginate Matrix, but the end result will always be bubbles. Do you have a recommended protocol for: 1. Making the alginate solution 2. Making the...
25 October 2021 2,509 6 View
Currently, I'm working on a DNA pol activity test, one of the variables is DNA pol concentration in the PCR/LAMP assay. For LAMP I found a clear protocol for the DNA pol in weights. However, for...
02 January 2021 10,021 3 View
In terms of vaccine solution, the vaccine (ex: protein sub-unit based) is contained inside nanoparticles (ex: PLGA). Does the adjuvant inside the nanoparticle as well or act as a solvent that...
04 October 2020 4,631 4 View
I'm a senior at a bioprocess major. I have a capstone course project about building a mini-plant for COVID-19 vaccine. The professor told me that the main point is the production aspect, not the...
03 October 2020 9,830 0 View
What is the function of thrombin in a plasmid? Does it help for screening, selection, or as a biomarker?
10 September 2020 1,730 3 View
Recently I have a group discussion about formulating a drug for Hepatocellular Carcinoma(HCC). My group agreed on using CRISPR/Cas 9 system to cure HCC, because theoretically it is more specific...
19 April 2020 6,149 0 View
Many publications mentioned that a Chitosan MW has a correlation with its antimicrobial activity (high MW = higher microbial activity). Is the reasoning solely that higher MW means a higher...
01 January 1970 2,281 11 View
Recently I've read a book about vaccine production. The book discusses Lemna minor and its advantages compared to algae. Mainly the secretion of metabolites. But It is rarely mentioned compare to...
01 January 1970 3,281 2 View
Could you share with me a reference for gorilla metabolism? Especially, their biosynthesis pathway of protein and muscle formation. Thank you in advance, stay healthy.
01 January 1970 6,615 3 View
