How to analytically describe a band pass filter Gaussian pulse?

I measure pulses on some background and my measurment system has an analog band-pass filter. I usually still have to do extra band-pass filtering digitally afterwards in order to remove the...

29 January 2020 3,494 4 View

Does anybody have an Android ".csv" Malware Data-set?

17 December 2019 761 3 View

What is a good RNA extraction method for RNA-Seq (especially QuantSeq)?

Which RNA extraction method would you recommend for RNA-Seq of snap-frozen fetal murine tissues? We will be doing Lexogen QuantSeq. Considering Qiagen RNeasy (good, but pretty expensive) or Thermo...

02 October 2019 9,527 3 View

Where can I find sizes of fetal mouse organs?

Does anyone know a resource for data on organ sizes (mass, preferably also dimensions) for fetal mice at various stages of development?

14 March 2019 2,229 1 View

Extracting RNA from FFPE tissue using Covaris/Bioruptor sonicator?

RNA extraction from laser microdissected FFPE tissues is markedly enhanced by sonication (paper below). However, I could only find a kit and protocol for Covaris sonicators. We only have a...

20 December 2018 2,932 1 View

How to extract bacterial DNA from swabs using bead beating?

We are happily extracting bacterial DNA using a bead beating protocol. Now we need to start processing swab samples containing fecal etc bacteria. If we put the entire swab into the bead beating,...

26 June 2016 333 4 View

Purifying a linearized plasmid?

I'm having trouble with contamination of linearized plasmid with the intact circular plasmid. I have heavily overdigested the plasmid, gel purified, and digested with another restriction enzyme,...

25 November 2013 1,907 5 View

Stability of sodium butyrate?

I'm using sodium butyrate in cell culture, but I'm slightly worried about the stability of my reagents. There is very conflicting information available regarding its stability in aqueous or...

10 March 2013 6,549 2 View

Tissue processing for laser microdissection and RNA isolation?

Hello! May I ask for your recommendations for tissue processing methods for laser microdissection and subsequent RNA isolation? I can think of at least the following protocols, each with...

12 February 2013 1,553 15 View

Low signal intensity in RT-qPCR (Cy3 probe, MX3005P)

I have problems getting very weak fluorescence signal from my probe-based RT-qPCR assay. The Ct is not bad (less than 30, and I see a clear although weak band on gel), but the signal intensity is...

17 January 2013 1,760 3 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

How to immerse bricks in wax ( for coating ) ?

Do we immerse the bricks horizontally or vertically ? #bricks#waterproofingsurface#civil#engeenering#chemicalengineering#wax#parafinwax

28 February 2021 6,685 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Why is my platinum counter electrode getting dirty during electrodeposition?

I am conducting anodic electrodeposition of MnO2 onto a stainless steel substrate, using a platinum strip as a counter electrode. This is done galvanostatically, with the current held between 5...

27 February 2021 1,829 3 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View