Hello.
My polyacrylamide gel becomes cloudy after de staining with a solution consist of (50% miliQ water + 40% methanol + 10% acetic acid) and the background of my gel does not become completely transparent.
I used Bio-Rad Coomassie Blue R-250 dye and I had just prepared the dye solution.
Thank you for your help.
I have attached an example of my gel scan.
It looks like there is some Coomassie Blue precipitate on the gel. Is your Coomassie freshly orepared and filtered before staining? If there is cloudiness besides the Coomassie, try destaining with ethanol rather than methanol.
This can be easily resolved by using freshly made de-staining solution (50% miliQ water + 40% methanol + 10% acetic acid): just heat the de-staining solution to 50 - 60 C degrees, put the gel into the solution, with gently shaking for about one hour, all the cloudy background will be gone.