Hanie Motahar

6 Questions 4 Answers 0 Followers

Questions related from Hanie Motahar

Alcian blue staining protocol for glutaraldehyde-fixed chondrocytes?

I have fixed human chondrocytes in 6-well plate by glutaraldehyde. For alcian blue staining pH 1, I used this protocol: 1) Rinse wells 3x with PBS. 2) Rinse 1x with 1% acetic acid. 3) Apply...

25 October 2023 7,362 0 View

Does anyone have experience extracting miRNA from serum?

Any suggestions for high quality miRNA extraction would be appreciated.

14 June 2023 5,908 3 View

Protocol for picrosirius red staining chondrocyte culture?

Hi, I fixed chondrocytes in 12-well plate with glutaraldehyde. I want to stain the cells by direct red. Do you have any protocol? Thank you.

05 February 2023 6,887 0 View

Is delta Ct=10 reasonable for TNF-a in OA cartilage?

Hi, I performed RT-PCR directly on the osteoarthritic cartilages obtained from 2 patients. In both experiments, Ct for TNF-a was found to be around 36, which is unexpected considering the...

01 February 2023 7,254 0 View

How can I fix chondrocytes before they undergo dedifferentiation?

Hi everyone, I want to fix osteoarthritic chondrocytes in 6-well plate with paraformaldehyde. Before fixation, it takes at least three days for the chondrocytes to adhere to the surface of the...

27 December 2021 9,278 4 View

What kind of phylogenetic tree will be suggested for a strain belonging to a new genus?

Dear all I isolated an archaeon with 93% similarity to the top-hit strain in 16S rDNA. First, I want to know which method is better to generate a phylogenetic tree, neighbor-joining or maximum...

09 September 2017 6,238 1 View