I am facing problems with transformation after successful transformation of the plant. But when I am introducing the plants for climatic adaptation the roots are dying. What may be the solution?
See i don't have much knowledge about tissue culture techniques but what i feel is that your culture might be lacking proper or even increased concentration of rooting hormones like cytokinins etc. You should have control callus with the experiment. If they are rooting normally then it might have something to do with the transformation, so try to increase the conc. of rooting hormones initially and if still it doesn't work then it could be some issue...but first check the control....
Regeneration of plants is pre-requisite for genetic transformation and legumes like chickpea is widely considered to be highly recalcitrant due to lack of in vitro induction and development of strong root system and establishment of in vitro raised plantlets in pots. So, transformation protocols which can bypass the tissue cultural procedures are required for transgenic chickpea development.
which legume you are using?
Many paper are published for in planta transformation of legumes particularly by Rohini et al 2011
I am too working with legumes in planta seed transformation.
Transformability in legumes is problematic as they are recalcitrant to regeneration(Thiruvengadam and Jayabalan 2000).
Tissue culture-in dependent in planta transformation would open new avenues in legume biotechnology (Keshamma et al. 2008a, b; Rao et al 2008).
regards.
Hi. Do the plants have good roots before removing them from media? I want to understand if you are facing a root regeneration problem or just acclimatation? I work with Medicago and acclimatation to ex vitro conditions is not a problem when plants have good radicular systems!
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