Does anyone know an efficient method to identify/sequence transposons insertion sites?

I am using Ez-Tn5 transposns system to create random mutations in E.Coli. After all the screening procedures I have narrowed down the samples I want to proceed for sequencing. However, I have...

30 March 2019 4,819 4 View

The crystallite size from XRD result of thin sheet under Scherrer's formula and thickness of the thin sheet should be same?

I made Indium Tin Oxide(ITO) thin layer and calculated crystallite size by using Scherrer's formula for XRD results. And I measured film thickness by using alpha step equipment and that value was...

21 August 2016 5,431 6 View

Is it possible to use Scherrer's formula to calculate crystallite size of spin coated thin film?

Is Scherrer's formula applicable to use for thin film X-RD results? If anybody knows or if somebody has theoretical details regarding physical relationship between the formula and crystal...

07 August 2016 6,625 5 View

What is happening when we anneal a spin coated ITO on glass substrate in a vacuum atmospheres up to 600C ?

In my ITO sol contains PVA as a binder/stabilizer. I did annealing and I got brown colored layer instead of transparent layer (After annealing my samples were not opaque but it was not good...

27 June 2016 9,676 7 View

What are the indicator species to have idea on climate change?

I think some of the lower plant species (fens, moss, etc...) can use as indicator species, but more infomations are not available for prove that idea.

23 December 2011 3,614 16 View

What is the best CPU for running Gromacs and Gaussian ?

Hello everyone. I am planning to build a workstation capable of running both gaussian and gromacs. I have planing to use RTX 3080 as the GPU and 64GB memory. However, I have not able to select a...

01 January 1970 7,229 2 View

How to validate and refine Protein structure (PDB cystalography data) ?

I am planing to do a docking analysis of protein 3VW6 using autodock. how can i validate and refine the crystallography structure ? I found these two web site claming to do so. can these sites...

01 January 1970 6,078 2 View

Why do i get a convergence error in gaussina DFT calculations eventhough the stationary state is found during geometry opt ?

Dear sir/madam, what is the reason for the above mentioned observation ? How can I eliminate it ? Thank you

01 January 1970 4,897 6 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

What are the application of Fixed points thoery in Chemistry.?

It is said that fixed point theory has lot of applications not in the field of mathematics only but also in various areas. So I am looking for applications in Chemistry only..

26 February 2021 3,533 1 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

How to interpolate from a time-series database ?

Hi, I am having trouble with a problem, in the field of Optimal Control and the generation of optimal time-series. Let's consider a system, whose dynamics are represented by dx/dt =...

24 February 2021 9,105 2 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View