That is way too general. There are nanoparticles that have size-specific absorptions and emissions (e.g. quantum dots, fullerenes, nanotubes) but that can't be generalized.
If you want to measure a particle size distribution, first you need to know where which particle absorbs, i.e. you either need an experimental calibration or at least a theoretical concept. For more details, refer to this discussion:
If you are concerned about the correctness of the results you obtain, based on uv-vis method i suggest you go for comparison of the results with an established method of nanoparticle size determination, using the two sample t test or in case you are taking replicate samples from a set of nanomaterials widely different in structure and properties, the paired t test.