Can someone share the protocol for calculating the extinction coefficient concentration of Silver Nanoparticles ?

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Does euthanasia by CO2 inhalation cause NF-kB activation in mouse heart or body in general?

I was wondering if euthanizing mice by carbon dioxide inhalation would cause activation of NF-kB inflammatory response?

26 January 2021 4,096 2 View

Can anyone suggest me solvent to dissolve EVA (Ethylene Vinyl Acetate) polymer?

I am working on experiment where i want EVA to dissolve in some solvent. can anyone suggest me solvent to use ?

06 January 2021 4,251 3 View

How to run Autodock 4 , using CONDA environment on server ?

#autodock #virtualscreening Question1: Am I doing this right , means do setting up conda on server works for virtual screening (AUTODOCK)? Question2: How can I modify the script (submit4.py)...

06 December 2020 2,424 3 View

Is it possible to isolate a His-tagged protein which has high prevalence of non-polar residues?

I am trying to express and isolate a protein in E. coli. The protein is supposed to have high prevalence of non-polar residues. Would that create problems in isolating the protein using a His tag...

08 September 2020 9,584 3 View

Can an isolated protein domain give a single band in SDS-PAGE equivalent to its dimeric mol. wt. ?

The protein I'm working with forms a homodimer and is a 2-domain protein. I have cloned and purified one of its domains. However, when I run the domain on SDS-PAGE, I get a single band equivalent...

19 August 2020 7,893 14 View

Can I prepare/download the dimeric structure of a protein which has a PDB file of the monomeric structure?

I want the dimeric structure of a protein (forms dimer) in .pdb format. The crystal structure of the protein is solved. However, the .pdb file at RCSB PDB is of the monomeric structure. Thanks...

09 July 2020 3,086 4 View

How to set the axis of fft2 in matlab for spatial wave?

I have some data of radiances over latitude and longitude. When we do fft, the frequency window is from -fs/2 to fs/2. But, when we do fft2, what will be in the x axis and y axis? -spatial...

16 November 2019 8,956 3 View

In DSP (digital signal processing) how can I retrive bits (data) after i have demodulated a signal to check BER (Bit Error Rate)?

Lets say, I have 10 randomly generated bits (0's and 1's) in matlab. From my other parameter calculations, I have about 5 samples, this means 1 bit/5 samples (each bit is replicated 5 times). My...

04 November 2019 6,514 3 View

What would be the best parameter to study a rising signal (say Calcium signals in live cells) ?

I am stimulating my cultured cells and recording calcium activity as fluorescence change using live-cell calcium imaging. Though I am more concerned about the decay of the signals, for which I am...

23 October 2019 3,842 6 View

Can anyone explain me why there is change in the Silver Nanoparticles UV-Vis max spectra before and after centrifuge ?

The final solution of AgNPs is pale yellow (I took the UV-Vis Spectra max) and then the solution was centrifuged thrice to obtain pure NPs i.e., dark colored (also took the UV-Vis Spectra max). So...

30 June 2019 7,758 5 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View