Hi. I use surfactant such as octyl b-D-glucopyranoside to solubilize liposomes. Mixing liposome dispersion and 1 M octyl b-D-glucopyranoside solution in equal volumes.

Keitaro Sou Hello. I have tried using surfactants but they form turbid phases or produce interferences during UV analysis (for example, they also absorb at 280 nm). Is the sae case with that surfactant? I don't only need to disrupt the liposomes but also mantain solubilized the protein without interferences

When the surfactant as well as liposome components have absorption at 280 nm, the absorption of the surfactant and liposome components without protein should be measured and subtracted as background for accurate determination. Otherwise suitable column chromatography may be considered to separate the components.

Avinash Singh Patel We are going to try ternary mixtures between chloroform:methanol and other solvents but I wanted to have some ideas to start.

Maybe introducing methanol to that mixture, and testing different proportions... It depends a lot on the formula and the protein hydrophilicity. We are now quantifying protein encapsulated in liposomes via SDS-PAGE but it's not that precise.

Quite practical topic. How about using Tween 20 or Triton X-100 to disslove the liposome?

Boxun Liu They interfere with the absorbance of the protein. I would like to employ some kind of organic solvent or mixture

Anyone knows about a mixture of organic solvents that would dissolve a hydrophilic protein? Thanks

David E. Bautista-Erazo Did you find something that works? I want to attempt something similar.

Benjamin Barlock Unfortunately, no :(. We are still using SDS-PAGE and densitometry to quantify protein encapsulation. It's very tedious.