What could cause non-specific backgrounds (probes) & DAPI staning in FISH?

In the procedures of FISH, are non-specific backgrounds (probes) and abnormal DAPI staning caused by : - Heat Pretreatment ; - Enzyme digestion ; or - Other? * Are these attached images represent...

04 May 2019 2,769 4 View

Storage of purified PCR product for sequencing ?

01 February 2019 296 6 View

Concentration of RNA for gene expression ?

I have isolated total RNA from FFPE tissues but the concentration is very low (1-2 ng/uL), the amount of 2-5 ng in reaction would work with the analysis of gene expression using sybrgreen in qRT-PCR ?

31 December 2018 8,434 5 View

Illumina sequencing data analysis and storage ?

In our institute, we are going to establish a Genomic platform (Illumina). For data analysis and storage, what are the minimum requirements in terms of "computer setting (HPC)" using sequencing...

01 February 2018 8,464 5 View

Conservation of blood samples for genetic analysis ?

02 March 2016 3,570 4 View

What are the minimum criteria for BSL-3 laboratory ?

31 December 2015 8,472 7 View

Which methods used to studying signal transduction pathways ?

02 March 2015 5,978 5 View

Is the use of a biological cabinet sufficient in a cell culture room (SP1)?

We are going to set up a new cell culture room. What are the necessary procedures to avoid contamination?

11 December 2014 4,310 6 View

Protocol to isolate manually DNA from FFPE samples ?

- Shord protocol to exctract DNA from FFPE samples

10 November 2014 8,512 0 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View