Are there simple and efficient methods for extraction both RNA and DNA from the same sample of Gram-positive bacteria?

14 January 2018 0 2K Report

I am working with highly mutagenized Bacilli species and wish to measure expression level of my favorite genes by real-time PCR. As I know, there are no reliable internal reference genes for Bacilli, and if there are, their expression may be significantly changed in the case of highly mutagenized bacteria (where one may expect changing in gene number). So, I think I can measure expression level of my genes relative to corresponding DNA fragments. To do so, I need a method for extraction of both DNA and RNA from the same bacterial culture. Can you suggest inexpensive, simple and efficient one?

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