Log phase of E.coli?

hii all, i am culture a E.coli bacteria with LB Broth, what the Appropriate condition that i can growth the E.coli (temperature, pH, Shaking, time) ? and how i can ensure that i have a E.coli in...

04 May 2018 1,482 3 View

Real Time Pcr?

hii everybody, how i can convert my Ct result that i got from Real time PCR to copy number that i can compare with CFU\ml result ? thank you very much Ammar

08 September 2017 7,773 0 View

Helicobacter pylori

hii everybody, i ask about all tips that can help me to grow this bacteria

07 August 2017 6,419 8 View

Gel electrophoresis (SDS-PAGE) ?

07 August 2017 3,528 13 View

Positive control as a plasmid - Real time pcr

06 July 2017 2,655 3 View

How i can know the "'gene copy number" according to the Concentration of the plasmid DNA for using as positive control on Q Real Time PCR analayize?

11 December 2016 2,257 1 View

How i can work with primer in Real Time PCR ?

Today i received a tetM primers forward and reverse and this is the first time for me to make a real time PCR reaction and i recived stock with 100(micro molar) that i need 218(micro liter) to get...

11 December 2016 3,588 3 View

Western blot for Candida Albicans ?

Hi everybody, 1) which is the best "buffer lysis" for Candida Albicans? 2) protocol for western blot that can be worked with streaked Candida albicans?

01 January 1970 5,994 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View