Antibiotic resistance genes in wastewater ?

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Log phase of E.coli?

hii all, i am culture a E.coli bacteria with LB Broth, what the Appropriate condition that i can growth the E.coli (temperature, pH, Shaking, time) ? and how i can ensure that i have a E.coli in...

04 May 2018 1,585 3 View

Real Time Pcr?

hii everybody, how i can convert my Ct result that i got from Real time PCR to copy number that i can compare with CFU\ml result ? thank you very much Ammar

08 September 2017 7,832 0 View

Gel electrophoresis (SDS-PAGE) ?

hi everybody, i work with gel electrophoresis ( SDS-PAGE) , when i loading the samples with the sample buffer*4 in the wells of the gel, the samples get away and get out from the wells, the...

07 August 2017 3,596 13 View

Helicobacter pylori

hii everybody, i ask about all tips that can help me to grow this bacteria

07 August 2017 6,470 8 View

Positive control as a plasmid - Real time pcr

hello, I have a 5`-3` forward and reverse primers and probe sequences for sul1 gene (sulfonamide resistance genes), ‎and i want to prepare a positive control as a plasmid that be appropriate to...

06 July 2017 2,753 3 View

How i can know the "'gene copy number" according to the Concentration of the plasmid DNA for using as positive control on Q Real Time PCR analayize?

about Real time PCR and positive control\ plasmid DNA how i can know the "'gene copy number" according to the Concentration of the plasmid DNA for using as positive control on Q Real Time PCR...

11 December 2016 2,333 1 View

How i can work with primer in Real Time PCR ?

Today i received a tetM primers forward and reverse and this is the first time for me to make a real time PCR reaction and i recived stock with 100(micro molar) that i need 218(micro liter) to get...

11 December 2016 3,643 3 View

Western blot for Candida Albicans ?

Hi everybody, 1) which is the best "buffer lysis" for Candida Albicans? 2) protocol for western blot that can be worked with streaked Candida albicans?

01 January 1970 6,055 4 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

Why does the MFDFA algorithm need to calculate the profile of the time series?

As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....

05 August 2024 9,366 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How can i do multivariate Time Series forecast using MLP, ANFIS and LSTM?

I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.

05 August 2024 2,977 3 View

Ali Akgul

Dear Ammar,

you can ask your question and wait responses. Everyone likes to help in here...

I like to help I can ...

Ali

Ammar Abou Kandil

Thank you ali

my question, is how i can make positive control for specific gene that i search for, for example, i have the primer and the probe for Sul1 gene and i want to make positive control for the Real time pcr analysis

Ammar