Can somebody share a standardised protocol for isolating phages against gram-positive bacteria
Hi, Niti Sarat Please check this out.
Article Complete genome sequence of a novel lytic bacteriophage, PLG...
Is there any correlation between the two and is there any research involving this?
16 November 2014 7,058 2 View
I use a 30bp Alexa labelled probe that binds to expanded repeats. The probe has good foreground signal but also binds to the nucleolus and membranes outside the nucleus and produces lots of...
04 March 2014 7,891 6 View
I am trying to do a transient analysis of wall structure (Steel Posts + Aluminium Panels) in ANSYS. For damping I have a value of logarithmic decrement 0.05. Could anyone tell me how I can use...
01 January 2014 5,397 3 View
I am to begin one such task and have decided on Bac as my cloning vehicle, and restriction digestion as the next step using BamH1 and pst1.
10 May 2012 3,295 4 View
The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....
27 February 2021 4,450 2 View
Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...
24 February 2021 9,839 3 View
Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
24 February 2021 3,157 6 View
I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...
16 February 2021 8,519 5 View
i am doing research on E.feacalis that is grown in dentin and after that, we did a gram stain for the sample my Q first does the gram stain consider as reliable tool for testing and seeing the...
14 February 2021 6,697 1 View
Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...
09 February 2021 4,994 1 View
My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....
08 February 2021 5,965 1 View
Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!
08 February 2021 6,909 3 View
When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...
05 February 2021 3,770 4 View
Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...
03 February 2021 4,630 10 View