09 December 2023 2 3K Report

Para ver proteínas, mas pequeñas

More Kary Leon's questions See All
How do I measure the virulence intensity of a fungus strain in a host plant?

I need to determinate how virulent is a fungus/oomycete strain-specie in comparative with others. I have plants group with and without fungus symptoms. However I found the fungus sources in both...

06 May 2024 8,974 5 View

How do resource shortages and discard rates from transfusion-transmissible infections influence the balance of blood supply and demand?

There are significant challenges that the blood bank industry in Kenya must overcome in order to be able to maintain a balanced equilibrium between the demand for blood and the supply of blood....

10 April 2024 974 2 View

What limitations were identified regarding the gold standard for HDV diagnosis?

Based on the study "Diagnostic Efficacy of Serological Antibody Detection Tests for Hepatitis Delta Virus: A Systematic Review and Meta-Analysis" by Zhenzhen Pan, et,al. (2023)

09 April 2024 1,796 1 View

What are the advances of the process of cleaning metallic threads on liturgic ornaments and clothes?

I wonder if there has been a progress in the way that conservators treat the metallic threads, especially the ones that are associated on liturgic textiles.

17 March 2024 4,773 0 View

¿Cómo puedo saber si una intervención con adolescentes problemáticos ha sido eficaz, o si por el contrario no ha sido adecuada?

Esta pregunta se refiere ha como podemos saber, si después de una intervención, ya sea en grupo o individualmente, a la hora de tratar con adolescentes problemáticos, nuestra intervención a sido...

11 March 2024 7,801 2 View

¿Cuáles son las principales acciones de respuesta del trabajo social, con respecto a menores en riesgo de exclusión social?

Esta pregunta hace referencia a las principales actuaciones, que un trabajador o una trabajadora social, debe de aplicar cuando se encuentra con un menor en riesgo de exclusión social, para que no...

11 March 2024 3,551 2 View

What steps should be taken to encourage primary care physicians in local communities to access national databases for antibiotic resistance?

This question is posted as it was stated in the study "Diagnostic Microbiology from the Beginning to the Future: Regional Antibiograms as Public Health Tools to Slow Down Antibiotic Resistance"...

05 February 2024 3,038 2 View

What stain technique can i use to visualize dinoflagellates P. Lunula?

Hello ! For my microbiology project i need to visualize living P.lunula under a lightmicroscope. I saw that you can try using Toluidine blue stain, but have not found much research about it.

16 January 2024 6,769 2 View

First singlet excited state optimizacion weird result?

Hello all, I hope someone here can help me with some TD-DFT calculations. I am trying to calculate the excited state optimised structures and energies of some Germanium compounds. Firstly, I run...

20 December 2023 4,593 2 View

Where can I buy pure lycopene extract or higher concentrations of lycopene in powder form?

Lycopene

13 December 2023 2,882 3 View

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When doing DNA extraction, what are the advantages of using Proteinase K over guanidine hydrochloride?

For many years I've been using a simple solution of guanidine hydrochloride and water as my lysis buffer when doing DNA extraction (both for arthropods and fungi). It's always worked fine for me...

01 October 2023 7,329 1 View

How proteinase k remain active in presence of denaturing agents??

I am trying to digest proteins by proteinase in blood samples for RNA purification. Suggested approach is to use SDS around 1% to denature proteins for better enzyme accessibility. would that...

11 April 2022 6,279 2 View

Does anybody have a good protocol for extracting DNA from mouse fecal pellets, without using a commercial kit?

Standard proteinase K lysis of fecal pellets does not seem to work. The main problem seems to be how to get rid of excess (fecal) material. Any suggestions will be very welcome. Thanks.

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What should I do if I don't get any band when checking the DNA concentration by viewing gel?

I checked the DNA concentration after I extracted the DNA of bacteria from plates. I picked colonies on plates and resuspended in PBS before adding the Proteinase K and Buffer. I already followed...

08 September 2018 2,359 9 View

How to remove dried cells/DNA off a nitex membrane for DNA extractions?

I'm working on developing a method to extract and isolate DNA from respiratory condensate or "blow" samples collected from beluga whales. In the field, we tried 2 different collection devices, a...

28 August 2018 5,043 2 View

Which is the best method for total DNA extraction from Lactobacillus spp. for qPCR?

I'm going to extract total DNA (both gDNA and plasmid DNA) from Lactobacillus (gram positive) for qPCR. So far I found some protocols using either glass-beads or enzymatic method (only for gram...

22 August 2018 6,871 5 View

How to remove dried cells/DNA off a nitex membrane for DNA extractions?

I'm working on developing a method to extract and isolate DNA from respiratory condensate or "blow" samples collected from beluga whales. In the field, we tried 2 different collection devices, a...

07 August 2018 4,574 3 View

PCR not working. Possible reasons?

I genotype and sequnce zebrafish using a bit of the fin. Lately though, I have been struggling with PCR. So far I have tried the following but to no avail: 1. Tried the primer pair in question...

07 August 2018 7,814 5 View

Which is the best method for total DNA extraction from Lactobacillus spp. for qPCR?

I'm going to extract total DNA (both gDNA and plasmid DNA) from Lactobacillus (gram positive) for qPCR. So far I found some protocols using either glass-beads or enzymatic method (only for gram...

07 August 2018 4,390 6 View

Has anyone used the Qiagen PowerFecal HT kit without a QiaCube ie via centrifuge? If so, how much buffer C4 did you add to 320ul from prior steps?

We should be able to use the Qiagen PowerFecal HT kit for 96 extraction of samples without the QiaCube robot, as the spin column/S-block assembly is the same as for the Plant 96 kit. However,...

06 July 2018 221 0 View

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