How to extract peak coordinates from a given cluster with MATLAB as in the SPM results viewer?

Hello everyone, I am trying to extract peak coordinates from given clusters in an 3D fMRI group image, e.g. a thresholded ICA z-score map. Using MATLAB/SPM I am able to extract a single peak...

26 January 2021 7,672 2 View

Is there any index number for first author in journal article like h index?

In every research paper, first author contribution is comparatively high. To tribute their work is there any index number for an author acting as first author in different papers?

15 December 2020 8,231 14 View

How to identify original journal website?

There are two websites for few journal with same journal title and same ISSN number. For example, There are two websites for the journal titled "High technology letters (ISSN: 1006-6748...

18 November 2020 7,650 6 View

Northern blotting advice?

Hi everyone! Does anybody know about a good website or place to get some guidance on Northern blotting? Also, advice on blotting the RNA ladder?

07 October 2020 739 3 View

Appropriate stats test for multiple DV, multiple treatments, paired across days?

Hi all, I'm trying to determine the right stats test for the project I'm working on. The setup is: 3 different treatment groups e.g. A, B, and C The same treatment is applied across different...

03 July 2020 4,487 7 View

Should there be sex education in schools and institutes about female menstruation?

02 July 2020 5,724 13 View

Do you have a specific question in glaucoma that may need a systematic review and/or a meta-analysis?

I have ophthalmology resident trainees who want to perform a systematic review and/or meta-analysis in glaucoma and want opinion from other experts about what topic seems relevant for them in this...

21 May 2020 6,692 1 View

Is there any correlation to relate temperature and shear strength of Aluminum alloy AA2024-T4?

As shear strength of AA2024-T4 alloy is inversely proportional to the temperature. is there any equation to predict shear strength of AA2024-T4 with respect to temperature?

12 May 2020 8,804 2 View

What is the protein composition of complete Freund's adjuvant?

The complete form of this adjuvant uses heat inactivated and dried mycobacteria. However, to rule out cross-reactivity with an antibody, I would like to know which proteins are left in the...

03 May 2020 1,332 2 View

What do you think about journal taking more than a year to publish a paper?

In this modern world technologies are updating too fast. In this scenario many journals take more than a year to publish a research article which may be an obsolete technology/process.

27 April 2020 9,394 11 View

Does anybody have a good protocol for extracting DNA from mouse fecal pellets, without using a commercial kit?

Standard proteinase K lysis of fecal pellets does not seem to work. The main problem seems to be how to get rid of excess (fecal) material. Any suggestions will be very welcome. Thanks.

09 October 2018 9,920 0 View

What should I do if I don't get any band when checking the DNA concentration by viewing gel?

I checked the DNA concentration after I extracted the DNA of bacteria from plates. I picked colonies on plates and resuspended in PBS before adding the Proteinase K and Buffer. I already followed...

08 September 2018 2,274 9 View

How to remove dried cells/DNA off a nitex membrane for DNA extractions?

I'm working on developing a method to extract and isolate DNA from respiratory condensate or "blow" samples collected from beluga whales. In the field, we tried 2 different collection devices, a...

28 August 2018 4,866 2 View

Which is the best method for total DNA extraction from Lactobacillus spp. for qPCR?

I'm going to extract total DNA (both gDNA and plasmid DNA) from Lactobacillus (gram positive) for qPCR. So far I found some protocols using either glass-beads or enzymatic method (only for gram...

22 August 2018 6,764 5 View

How to remove dried cells/DNA off a nitex membrane for DNA extractions?

I'm working on developing a method to extract and isolate DNA from respiratory condensate or "blow" samples collected from beluga whales. In the field, we tried 2 different collection devices, a...

07 August 2018 4,556 3 View

Which is the best method for total DNA extraction from Lactobacillus spp. for qPCR?

I'm going to extract total DNA (both gDNA and plasmid DNA) from Lactobacillus (gram positive) for qPCR. So far I found some protocols using either glass-beads or enzymatic method (only for gram...

07 August 2018 4,321 6 View

PCR not working. Possible reasons?

I genotype and sequnce zebrafish using a bit of the fin. Lately though, I have been struggling with PCR. So far I have tried the following but to no avail: 1. Tried the primer pair in question...

07 August 2018 7,657 5 View

Has anyone used the Qiagen PowerFecal HT kit without a QiaCube ie via centrifuge? If so, how much buffer C4 did you add to 320ul from prior steps?

We should be able to use the Qiagen PowerFecal HT kit for 96 extraction of samples without the QiaCube robot, as the spin column/S-block assembly is the same as for the Plant 96 kit. However,...

06 July 2018 145 0 View

Saliva DNA Extraction Incubation Time?

Hello, I am currently working through a set of saliva samples for DNA extraction. We have been following a protocol that begins by combining the sample with 60 ul of Proteinase K and 300 ul of...

21 June 2018 4,522 2 View

Saliva DNA Extraction Incubation Time?

Hello, I am currently working through a set of saliva samples for DNA extraction. We have been following a protocol that begins by combining the sample with 60 ul of Proteinase K and 300 ul of...

05 June 2018 2,325 3 View