So far I've got very mixed opinions on the matter. I cannot use liquid nitrogen or beads for homoginzation, thereby, would it be ok to use vortex to homogenize tissue? I duobt that it would damage the rna.
Vortexing is safe, less prone to contamination and easy to perform. However, vortexing may not be sufficient to homogenize some types of tissues, such as collagen-rich or elastic tissues, that require more mechanical force to break down . Vortexing may also cause heat generation and foaming, which can affect the quality and yield of RNA .
Therefore, it is recommended to use vortexing in combination with other methods, such as freezing and thawing, sonication, or enzymatic digestion, to improve the homogenization efficiency and RNA integrity. It is also important to optimize the vortexing time, speed, and temperature, and use appropriate buffers and stabilizers to protect the RNA from degradation .
If you want to learn more about vortex to homogenize tissue, you can check out these web search results:
- Guide to the Homogenization of Biological Samples - OPS Diagnostics LLC
- Tissue Homogenization - KSU Faculty
- Tissue Homogenization Techniques for RNA Extraction
- Tissue Homogenization and RNA + Protein Extraction using TRIzol reagent …
- A novel method of sample homogenization with the use of a microtome …
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