To be developed.
I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...
22 February 2021 6,937 3 View
Hello everyone, I got a plasmid containing E. coli, and its description on the tube is Bacterial stab (DH5a)_HlZ31_cr_Amp_, I know that Bacterial stab refer to LB medium, DH5a is the name of E....
14 January 2021 6,326 2 View
I am working with Balb/c mice, and in my experiment, I have to inject mice with antigens, then get their spleen and collect the splenocytes for fusion. Recently, I get into trouble with the mouse...
04 January 2021 3,591 3 View
I am looking for articles, coferences, publications or thesis concerning the capture of solid oleophilic polymers in solution in water. Thanks Thierry Le Blan
01 January 2021 5,798 3 View
Hello everyone, I got a partial cds, and I want to express it in yeast, does it work with partial cds or I need a complete one? If partial cds is possible, can you please suggest me some articles...
27 December 2020 3,688 3 View
Dear scholars, Our research is currently involved with hybridoma technology. We have isolated some original clones after fusion and we are preparing the subclone step to find some true positive...
01 December 2020 1,178 3 View
Dear all, I want to choose a normal ovary cell line and check for its proliferation property when introducing carcinogenic factors. Does anyone have any suggestions for the cell line? Many thanks...
08 November 2020 9,100 3 View
Dear all, I find a paper using Snapback primer. I do not understand the function of snapback tail? What is snapback primer? When do we use snapback primer? and why we use that? Thank you.
01 November 2020 8,869 2 View
22 October 2020 9,354 4 View
I am planning to characterise the transcriptome of pre-anthesis kiwifruit anthers. The samples are being harvested on my behalf and sent down by post, and I cannot ask the harvesters to dissect...
10 October 2020 1,830 3 View
We are trying to run fiber photometry experiments for long time scales, but we get these sinusoidal artifacts that come up. We think they may be caused by twisting of the fiber optic subject...
25 February 2021 568 1 View
As Cronbach's Alpha is usually used for test the reliability of measurements given by questionnaires, however, I wonder that whether I use this test for features extracted from behavioral data. I...
26 December 2020 9,602 2 View
everyone knows the importance of knowledge but knowing this too why there is a restriction in getting access to it what is reason behind this.
23 December 2020 2,934 6 View
I have submitted a paper in a journal and the reviewers reply to me with this. Can anyone guide me on this how to solve it Thanks in advance Figure 4: (i) the image presented in panel (a) shows...
15 December 2020 636 6 View
Sorry, I have witnessed the Peter Principle running rampant in corporations and running twice as rampant in the academic world. Please explain.
11 November 2020 8,240 2 View
What is the best method of avoiding plagiarism when writing a publication, thesis or dissertation in academia?
20 October 2020 9,322 23 View
Dear all, Can anyone tell me free Scopus indexed journals for publishing a Review paper on EEG signal artifacts removal methods?
19 October 2020 7,798 3 View
Most of my microsatellite markers are dinucleotides, therefore they show stuttering. In regular stuttering is easy to identify the allele, which is the highest peak on the right, after the shorter...
26 September 2020 1,486 5 View
I am processing data in Kubios HRV Standard and I am running into two technical issues. I am specifically analyzing HR and changes in nonmotor HR, and I'm finding that most of the information...
20 September 2020 1,651 1 View
In finite element method, especially when developing new elements, we often need integration techniques. Generally, numerical integration is used, however, in order to enhance the accuracy of our...
12 August 2020 997 19 View
