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I am validating the expression of several genes by qPCR to confirm transcriptome analysis. After many inconclusive runs, I realised my housekeeping gene (GAPDH) was downregulated in my...
28 June 2024 7,615 3 View
I have molecules with different groups. Some are bis chalcones, some are Dicoumarins, etc. How do I align them in Sybyl software if I can't select any common template?
28 June 2024 7,390 1 View
Hello everyone, I'm searching for team research interested to work with: An innovatif product has many interesting therapeutic activities and needs to be devellopped. For example i share with you...
28 June 2024 9,767 2 View
In an antibiotic degradation experiment using metals carbon composites to activate PMS, the results doesn't fit pseudo first or second order kinetics however the degradation efficiency is very...
27 June 2024 7,029 3 View
I'm trying to obtain MSCs from whole blood PBMCs and from the CD34- fraction of umbilical cord blood. However, I'm obtaining other types of cells that prevent the MSCs from growing. These cells...
27 June 2024 3,982 1 View
I am using a vector that has repeat sequences, and using Mach1 competent cells. I heard that if the cells are not stable cells, then we shouldn't grow them in 37 to avoid recombination to happen...
27 June 2024 4,029 3 View
I am polishing with emery papers with different grit sizes followed by diamond polishing and colloidal sols after ultrasonication with no scratch After that when I go to EBSD CI is not coming....
26 June 2024 2,509 3 View
Hello, I've just attempted a wet transfer of a western, and had set up the cassette and tank, and run @ 100V and set it to 90 mins. I had pre-chilled the transfer buffer - but didn't put the...
26 June 2024 7,493 2 View
The line appears always in the penultimate run. We try to decrease the volume of the solvent system, wich is composed of butanol:acetic acid:water (2:1:1) (76 mL) but the line remains. Below I...
25 June 2024 9,923 3 View
Hi, Does anyone know pet28 cloning vecot can express in E.coli? I have to clone two genes in to pseudomonas and I dont know whether pet28 plasmid derived from E.coli can whould be successful. On...
25 June 2024 5,396 2 View
I've been doing transformation several times with DH5. I got overgrowth/bacterial lawn growing on my plates twice, and when I transform for the 3rd time, there's no colonies formed. I'm using heat...
23 June 2024 4,938 1 View
I want to confirm the information so I can add it to my simple information
21 June 2024 4,240 0 View
If I want to increase the photocatalytic activity of a thin film sample what should be it's ideal surface morphology and parameters? All scientific answers related this are highly appreciated.
20 June 2024 6,902 0 View
Hello, I recently uploaded papers. when I had to add co authors, their profile didn't appear when I began to type their name (I tried with or without uppercase). They are then not connected to my...
20 June 2024 5,978 1 View
Clinicians need to crimp the self-expandable stents into a delivery sheath so they can deploy it at a target site. I want to know if there are any researchers out there discussing the amount and...
18 June 2024 5,355 0 View
I am seeking a collaborator for research in q-calculus.
17 June 2024 9,118 4 View
I have a target of sequences for primer design for qPCR with taqman. The issue is that when I design a sense probe the probe makes dimers with forward and reverse with a very high probability at...
17 June 2024 1,591 1 View
Comprehensive Overview of Myocardial Infarction: Etiology, E... What are the primary modifiable risk factors for myocardial infarction identified in the INTERHEART study?
16 June 2024 5,437 1 View
Comprehensive Review of Thrombolytic Therapy: Mechanisms, Ap... How should thrombolytic therapy be monitored, and what steps should be taken in case of adverse events?
16 June 2024 864 1 View
Comprehensive Overview of Myocardial Infarction: Etiology, E... How has the incidence of myocardial infarction changed in recent decades in the United States?
16 June 2024 3,381 1 View
I need to conduct the Soxhlet extraction method to isolate piperine from black pepper. Are there any strategies to optimize the yield of the extracted piperine?
15 June 2024 6,584 3 View
"Hi, I'm cloning a 700bp gene into pCAMBIA 1304 and transforming DH5-alpha cells. Colonies appear on selective plates, but in broth, I see thread-like structures and can't isolate plasmids. Any...
15 June 2024 5,163 2 View
how avoid forming lumps in disolving Laemnili buffer 5x? due to presence of undissolved bromoclesol blue and thick glycerol. Please tell me how to resolved this issue.
13 June 2024 10,088 3 View
Hello What are the experiences and perspectives of nurses providing nursing care at home and in the community for patients in palliative care?
13 June 2024 2,368 5 View