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Reductionist approach of science vs unexplored areas like strong emergence and consciousness ?

As AI can take care of the reductionist approach of science very well, can humans concentrate more on unexplored areas like strong emergence and consciousness?

17 May 2024 4,118 0 View

Why my NiSO4(H2O)6 is not dissolving in 1X PBS buffer solution?

I am trying to recharge the Ni-NTA column for his-tagged protein purification but my NiSO4(H2O)6 is not dissolving in 1x PBS and I have tried it from a different reagent bottle also, please help!

16 May 2024 9,892 3 View

SPME: Why is this happening with the SPME fibers?

I have been using a plain gray SUPELCO SPME fiber for direct immersion in 1% KOH, and in one recent occasion, I left samples running (around 10), and I came back to a broken fiber. I tried to...

16 May 2024 7,105 2 View

Do you recommend any transportation software for simulating crowd-based transportation systems like crowdshipping?

I've been looking for software that simulates the integration of passenger and freight transportation systems. For instance, tools like Vissim and Visum primarily focus on modeling passenger...

15 May 2024 9,479 2 View

How can I publish my research article here?

I wrote 3 articles on coaching talents from Football to Business and what the correlations and impacts are.

15 May 2024 3,982 4 View

During variable selection to enter into the multivariable analysis, is that a must to run a bivariable analysis when the number of variables are < 10?

I want to select variables to enter into the multivariable analysis by doing a bivariable analysis but the number of variables are limited,so is there any ground rule in such condition?

14 May 2024 7,960 1 View

GC-MS calibrated for 16 EPA PAHs. Last 3 compounds show poor linearity. Response decreases. How to improve?

I have calibrated my GC-MS for 16 EPA PAHs using available methods. However, the last three compounds (Indeno[1,2,3-c,d]pyrene, Dibenzo[a,h]anthracene, and Benzo[g,h,i,]perylene) are not showing...

14 May 2024 5,167 2 View

How to properly extract proteins from a Caco-2 cell line?

Hi everyone! As I previously shared with you, I've started to work with Caco-2 cells and even asked for your help. I recently followed a protocol to extract proteins, but when I collected the...

13 May 2024 6,969 2 View

English reading comprehension test?

Hi Research Gate community, I'm conducting an intervention study and am in need of a brief instrument to measure English reading proficiency. Many studies employ standardized tests like the TOEFL...

13 May 2024 973 4 View

Does epiphytic orchids establish relation with fungi when germinate?

Terrestrial orchids establish a relationship with fungi when germiate. Duw to their small size, they lack reserve substances and need a fungus to feed them, a relationship that can last the entire...

13 May 2024 1,695 2 View

Is there any formula which is used to calculate the value of capacitive contribution in the case of battery type electrode?

Dunn's equation should not be used in the case of a battery-type electrode, according to a paper published in the journal "Phys. Chem. Lett. 2020, 11, 9846–9849 (ref. attached)" that I recently...

13 May 2024 3,643 0 View

How can enzyme engineering techniques such as directed evolution or rational design be applied to improve catalytic activity and stability?

Enzyme engineering techniques can be applied to improve catalytic activity and stability of the bio catalyst. Examples: -directed evolution -rational design etc.

13 May 2024 7,816 0 View

How can I teach children IP?

I am interested in materials that can help teach and guide kids on IP

12 May 2024 6,489 7 View

What are the strategies for optimizing biocatalysts for carbon conversion?

For carbon conversion , the use of biocatalysts must be optimized, so what are the strategies used for this purpose?

11 May 2024 8,001 1 View

Is there any way to determine the concentration of protein in a gel-ready protein sample?

I am performing western blot and recently i have been obtaining faint bands for the samples i had already run and had got darker bands. I wish to determine the concentration of protein in those...

09 May 2024 9,102 4 View

Dear Prof and Dr, how to calculate the recoverable energy density from P-E hysterisis loop ?

The recoverable energy density can be evaluated by the following equation: Wrec = ∫P EdP = Area CDBCJcm−3 (4) Thus, when domains are realigned by the applied electric field, the difference...

09 May 2024 4,727 0 View

Are there genetically engineered soybean seeds that germinate at different rates?

I read about a line of seeds that germinate at different times to ensure a crop despite drought, but I can't find the name of the seed.

09 May 2024 2,546 2 View

Discovering a proof for Goldbach's Conjecture ?

FOR ME incredible! Discovering a proof for Goldbach's Conjecture is a monumental achievement in the realm of number theory. I perseverance and insight have unlocked a solution to one of...

09 May 2024 4,666 6 View

What is the identity of this E. coli protein that ends up in the insoluble fraction/ inclusion bodies?

I am purifying different proteins that go into inclusion bodies when expressed in E. coli. When I purify my inclusion bodies I always find two bands around 37 kDa in my solubilized fraction. These...

07 May 2024 6,455 2 View

What is the meaning of depth and level in Imaris statistic analysis (filament function)?

I used filament tracing of Imaris to reconstruct dendritic spine and I got the statistic result of dendritic spine density. I wanted to count dendritic spine density in secondary and tertiary...

07 May 2024 8,824 0 View

Which cirteria using evaluates usage of digital transfromation technology at the international airport?

I need evulation criteria for usage of digital transformation at the international airport. If someone has related material and resource please share me. Thank you.

07 May 2024 7,224 0 View

Is expert panel indispensable for pedagogical framework & scale development?

Hello everyone, Thank you for your time and patience in advance! I attempt to develop a pedagogical framework and a competence scale for my PhD project. I have developed the drafts of both. I...

07 May 2024 2,368 3 View

Hello,I prepared MgO NPs by sol-gel method, for raman spectroscopy what range should i use? because i used (100-900) but there is no peak?

Actually, I prepared a large amount of MgO, after aging I kept it in a hot air oven at 120 for 12 hours, but there was still moisture there, then I kept it again in a hot air oven at 120C for 6...

06 May 2024 6,433 2 View

Is it appropriate to test presence/absence effects through a factorial design?

For factorial designs I usually see the independent variables being tested in levels (e.g., high/low). In my experiment, though, the effects of two different independent variables can only be...

06 May 2024 4,278 1 View

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