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Can I detect endogenous RNA in human plasma samples?

I would like to implement a endogenous RNA-based internal amplification control for a RT-qPCR assay used to detect viral pathogens in human plasma samples. Are there any detectable endogenous RNA...

23 May 2024 1,890 1 View

Can AI solve global warming and hat role can technology play in addressing climate change from innovations in clean energy to carbon capture &storage?

Can AI solve global warming and hat role can technology play in addressing climate change from innovations in clean energy to carbon capture and storage?

22 May 2024 1,706 5 View

Doesquarter of globalharvests at risk if agriculture does not adaptto climatechange&effect of climatechange will prompt changes inagricuturalpractice?

How is a quarter of global harvests at risk if agriculture does not adapt to climate change and effect of climate change will prompt changes in agricultural practices?

22 May 2024 2,778 5 View

What are the strategies to mitigate climate change impact on agriculture and how to reduce the impact of climate change on agriculture in India?

22 May 2024 4,793 5 View

How can climate change adaptation strategies be integrated into agricultural policies & practices at local, national, & global levels & global levels?

How can climate change adaptation strategies be integrated into agricultural policies and practices at the local, national, and global levels?

22 May 2024 2,740 8 View

What are the adaptation and mitigation strategies for climate change in agriculture & impact of climate change on agriculture production importance?

What are the adaptation and mitigation strategies for climate change in agriculture and impact of climate change on agriculture production importance?

22 May 2024 2,341 5 View

Could the small transmembrane domain of the recombination protein from the virus be the reason for the lack of expression in E. coli?

I have a protein that is 117 amino acids long, with a 21 amino acid transmembrane domain at the C-terminus. I've cloned this protein into the pET28 expression vector in BL21, but am not seeing any...

21 May 2024 8,137 2 View

Errors with TD-DFT using PBE0?

Running into an error when trying to do TD-DFT PBE0 functional and DGDZVP as basis set. Error reads as follows: 'No CIS or TD with Post-SCF methods' Input Line as follows: #p PBE0/DGDZVP...

21 May 2024 5,968 4 View

How to use microbes to remediate soil contamination quickiy, efficiently and cheaply?

Be curious and want to learn something from cases

21 May 2024 5,046 4 View

How can biocatalysis be integrated into larger carbon conversion processes, such as biorefineries or waste valorization schemes?

biocatalysis can be integrated into larger carbon conversion processes, such as biorefineries or waste valorization schemes. The main question: HOW

19 May 2024 2,836 0 View

What are the environmental benefits of using biocatalysis for carbon conversion compared to traditional chemical methods?

The environmental benefits of using biocatalysis for carbon conversion compared to traditional chemical methods are large. What are they?

19 May 2024 3,585 0 View

How empathy can be quantified ?

In what ways can empathy be measured or assessed to determine its extent or impact, and how can its presence or level be quantified through observable behaviours, physiological responses, or...

18 May 2024 4,228 3 View

Are You Interested in Collaborating on Chemistry Research?

Hello ResearchGate Community, I am an MS Chemistry student with a keen interest in advancing my research skills and contributing to impactful projects. As I embark on my journey in the world of...

18 May 2024 3,192 4 View

What happens to frequency and wavelength when temperature increases and relationship between the speed of light, frequency, wavelength and period?

18 May 2024 8,513 0 View

Reductionist approach of science vs unexplored areas like strong emergence and consciousness ?

As AI can take care of the reductionist approach of science very well, can humans concentrate more on unexplored areas like strong emergence and consciousness?

17 May 2024 3,958 0 View

Why my NiSO4(H2O)6 is not dissolving in 1X PBS buffer solution?

I am trying to recharge the Ni-NTA column for his-tagged protein purification but my NiSO4(H2O)6 is not dissolving in 1x PBS and I have tried it from a different reagent bottle also, please help!

16 May 2024 9,723 3 View

SPME: Why is this happening with the SPME fibers?

I have been using a plain gray SUPELCO SPME fiber for direct immersion in 1% KOH, and in one recent occasion, I left samples running (around 10), and I came back to a broken fiber. I tried to...

16 May 2024 6,895 2 View

Do you recommend any transportation software for simulating crowd-based transportation systems like crowdshipping?

I've been looking for software that simulates the integration of passenger and freight transportation systems. For instance, tools like Vissim and Visum primarily focus on modeling passenger...

15 May 2024 9,257 2 View

How can I publish my research article here?

I wrote 3 articles on coaching talents from Football to Business and what the correlations and impacts are.

15 May 2024 3,842 4 View

During variable selection to enter into the multivariable analysis, is that a must to run a bivariable analysis when the number of variables are < 10?

I want to select variables to enter into the multivariable analysis by doing a bivariable analysis but the number of variables are limited,so is there any ground rule in such condition?

14 May 2024 7,793 1 View

GC-MS calibrated for 16 EPA PAHs. Last 3 compounds show poor linearity. Response decreases. How to improve?

I have calibrated my GC-MS for 16 EPA PAHs using available methods. However, the last three compounds (Indeno[1,2,3-c,d]pyrene, Dibenzo[a,h]anthracene, and Benzo[g,h,i,]perylene) are not showing...

14 May 2024 4,939 2 View

How to properly extract proteins from a Caco-2 cell line?

Hi everyone! As I previously shared with you, I've started to work with Caco-2 cells and even asked for your help. I recently followed a protocol to extract proteins, but when I collected the...

13 May 2024 6,789 2 View

English reading comprehension test?

Hi Research Gate community, I'm conducting an intervention study and am in need of a brief instrument to measure English reading proficiency. Many studies employ standardized tests like the TOEFL...

13 May 2024 823 4 View

Does epiphytic orchids establish relation with fungi when germinate?

Terrestrial orchids establish a relationship with fungi when germiate. Duw to their small size, they lack reserve substances and need a fungus to feed them, a relationship that can last the entire...

13 May 2024 1,530 2 View

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