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I am polishing with emery papers with different grit sizes followed by diamond polishing and colloidal sols after ultrasonication with no scratch After that when I go to EBSD CI is not coming....
26 June 2024 2,422 3 View
Hello, I've just attempted a wet transfer of a western, and had set up the cassette and tank, and run @ 100V and set it to 90 mins. I had pre-chilled the transfer buffer - but didn't put the...
26 June 2024 7,437 2 View
The line appears always in the penultimate run. We try to decrease the volume of the solvent system, wich is composed of butanol:acetic acid:water (2:1:1) (76 mL) but the line remains. Below I...
25 June 2024 9,846 3 View
Hi, Does anyone know pet28 cloning vecot can express in E.coli? I have to clone two genes in to pseudomonas and I dont know whether pet28 plasmid derived from E.coli can whould be successful. On...
25 June 2024 5,323 2 View
I've been doing transformation several times with DH5. I got overgrowth/bacterial lawn growing on my plates twice, and when I transform for the 3rd time, there's no colonies formed. I'm using heat...
23 June 2024 4,856 1 View
I want to confirm the information so I can add it to my simple information
21 June 2024 4,175 0 View
If I want to increase the photocatalytic activity of a thin film sample what should be it's ideal surface morphology and parameters? All scientific answers related this are highly appreciated.
20 June 2024 6,759 0 View
Clinicians need to crimp the self-expandable stents into a delivery sheath so they can deploy it at a target site. I want to know if there are any researchers out there discussing the amount and...
18 June 2024 5,300 0 View
I am seeking a collaborator for research in q-calculus.
17 June 2024 9,093 4 View
I have a target of sequences for primer design for qPCR with taqman. The issue is that when I design a sense probe the probe makes dimers with forward and reverse with a very high probability at...
17 June 2024 1,521 1 View
Comprehensive Overview of Myocardial Infarction: Etiology, E... What are the primary modifiable risk factors for myocardial infarction identified in the INTERHEART study?
16 June 2024 5,381 1 View
Comprehensive Review of Thrombolytic Therapy: Mechanisms, Ap... How should thrombolytic therapy be monitored, and what steps should be taken in case of adverse events?
16 June 2024 828 1 View
Comprehensive Overview of Myocardial Infarction: Etiology, E... How has the incidence of myocardial infarction changed in recent decades in the United States?
16 June 2024 3,298 1 View
I need to conduct the Soxhlet extraction method to isolate piperine from black pepper. Are there any strategies to optimize the yield of the extracted piperine?
15 June 2024 6,523 3 View
how avoid forming lumps in disolving Laemnili buffer 5x? due to presence of undissolved bromoclesol blue and thick glycerol. Please tell me how to resolved this issue.
13 June 2024 10,043 3 View
Hello What are the experiences and perspectives of nurses providing nursing care at home and in the community for patients in palliative care?
13 June 2024 2,255 5 View
I conducted a tensile test to analyze the strength of a welded joint and to implement an FEM model. Due to the high hardness of the base material, slippage between the grip and the base material...
11 June 2024 5,773 6 View
The buffer wasn't filtered.. Could this be the cause? Can we autoclave phosphate buffer to resolve this issue?
11 June 2024 3,989 2 View
Dear professors and colleagues， hello！Recently, while studying the CDP model, I have read the manuscripts of various experts and have gained a lot, but I still have some questions.What I...
11 June 2024 1,925 1 View
I am optimizing a multi-stage optimization model for disaster response relief delivery. In this regard, I want to know the realistic values of cost parameters related to the operating and setup...
11 June 2024 4,257 3 View
I've pelleted down the overnight ampicillin supplemented cultures of DH5ALPHA for pET22b isolation 5 days ago and stored them on -20°C without adding any buffer or glycerol. Would the cells be...
10 June 2024 6,495 2 View
Hey guys! My name is Lígia and I work with GasBench II/IRMS (Delta V Advantage). We have a problem with our instrument. The water recorded by m/z 18 is around 20V. We checked the Nafions and they...
10 June 2024 1,083 4 View
Methodological Rigor of Review Articles in Medicine What factors contribute to the value of a review article, and how is this value related to scientific criteria?
06 June 2024 2,862 1 View
How does epinephrine exert its pharmacologicaleffects? Comprehensive Clinical Applications and Management of Epinep...
06 June 2024 7,725 1 View