I've been doing transformation several times with DH5. I got overgrowth/bacterial lawn growing on my plates twice, and when I transform for the 3rd time, there's no colonies formed. I'm using heat shock method with chemically synthesized plasmid and ampicillin as the marker. The plates i'm using is only several days old and i cover it with amp before plating the transformants. what should i do to increase my TE?
Akashata Dawane
Use fresh ampicillin plates or use carbenicillin in place of ampicillin.
If you are using 100 ul of DH5a then use 50 ul for transformation but before that try fresh plates. Make it the same day of transformation
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