Hello, I've just attempted a wet transfer of a western, and had set up the cassette and tank, and run @ 100V and set it to 90 mins. I had pre-chilled the transfer buffer - but didn't put the chamber in an ice bucket/add ice-packs (this is my first time attempting a wet transfer, I'm used to using semi-dry). When I walked in after 30 mins, the tank was genuinely steaming and boiling to touch. I switched it off and took out the gel/membrane, and am now blocking the membrane in some vain hope that there's something on it (but doubt it with the overheating and the fact it was only transferring for 30 mins). Has anyone had any experience of this - and it worked? And is there anything else you'd suggest other than setting the chamber up in an ice bucket?
I'm not surprised by the results, we also used wet-transfer method and used ice buckets filled with ice (https://x.com/MacAdemics_Ed/status/1070787991893929984/photo/1) to keep the temperature down. Do you have a magnetic stirrer in the transfer buffer as well? That will help as well.
you need to bring the temperature down as the wattage used in wet transfer generates a lot of heat. place the rig and power pack in a cold room or refrigerator and put a top a stir plate to circulate the buffer.
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