There seems to be a problem with virus production using PlatA cell. If there is no problem with retroviral vector cloning but a stable cell line is not made, what should I check? Should I check the virus titer?
Taufeeque Ali
Measure virus titer (via qPCR, infectivity assays, etc.). Optimize transfection conditions for Plat-A cells. Verify helper plasmids and their expression. Ensure Plat-A cell health (passage number, culture conditions). Check viral supernatant quality. Confirm target cell line susceptibility to retrovirus. Ensure proper selection conditions for stable cell line generation. Check vector integrity and design.
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