Dear experts it may concren,
We want to use stereotaxic injunction technique give AAV for up-regulating a protein in whole cerebella in P0 mice. Firstly, we have to try different injection locations and volumes for whole cerebella being infected. Normally, we should wait 4w to see the virus expression. Do you know someways to visualize AAV spreading area in 12h or 24h for saving time and reducing unnecessary harm to more mice ?
Thank you!
If you inject the AAV into the entire cerebellum at P0, expression can typically be observed between P3 and P5. Simply place the pups under a fluorescence microscope to visualize it. However, the visibility also depends on the type of promoter used in your AAV.
Thank you so much for reply Winda Ariyani , have you ever injected AAV into whole cerebellum at P0? i am finding one expert who had the experience injecting AAV into whole cerebellum at P0, cause i am totally new in P0 mice, even have no ideas fixing them and measuring the coordinates.
Not in the cerebellum, but I have injected AAV in the PFC and S1 cortex at P0.
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