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Questions related to Organic
How do newer HPLC methods compare with post column ninhydrin detection on an ion exchange column?
04 October 2022 9,297 2 View
We have a Q-exactive Orbitrap mass spectrometer. I have noticed that the number of protein groups observed from 100 ng of HeLa whole cell lysate (purchased from Thermo) has gone down from 1800 to...
27 September 2022 6,736 3 View
Is there a way to download all the files of protein-ligand complex in pdb? Or anyone known a database of protein-ligand complex ready to use?
26 September 2022 6,105 0 View
Which method is better to analyze for example Vit D and its metabolites?
24 September 2022 2,074 4 View
how can I convert glycine methyl ester into glycine ?
22 September 2022 2,209 3 View
looking for research title that related to medical laboratory, that will also provide the experimental that will present in the laboratory. Thank you!
12 September 2022 2,618 1 View
Hi there, I'm currently doing research on the compsition of the liquid phase generated by thermal degradation of Poly(methyl methacrylate) (PMMA). In my group we are especally interested in...
09 September 2022 9,474 1 View
In a 19F-NMR spectrum, the chemical shifts are usually negative. Therefore, does "upfield" become leftward movement towards zero, or does it still refer to rightward movement, but now becoming...
06 September 2022 1,839 6 View
Can plz anyone help me to calculate J-coupling constant for the given NMR spectra?
06 September 2022 2,662 3 View
Hi, I've previously run 3D NMR experiments on a labelled protein using the Biopack options in VNmrJ and then processed my data using CcpNmr. The experiments went well and I'm happy with my...
05 September 2022 7,758 0 View
How do organisms break down organic matter in the soil and sustaining soil health? What’s relationship between organic matter and soil fertility?
31 August 2022 9,281 4 View
Hello dear friends, I've recently started studying about wastewater treatment and would like to write a research proposal about sludge recycling as fertilizer. So, I was wondering whether it is...
28 August 2022 1,120 6 View
In the process of protein-ligand complex formation, some ligand/protein will not form complexes in the solution, so how to remove free ligand/protein? how pure is the protein-ligand complex sample?
27 August 2022 2,537 1 View
To purify the protein, I am using 0.5% Triton X-100 because it is hydrophobic. Lysis buffer has ne contain imidazole and elution buffer has 250 mM imidazole. I tried to optimize imidazole...
18 August 2022 9,449 8 View
What information do the blue(positive contour) and green(negative contour) tell me in HSQC? Can I discover any information about a structure depending on if the contour is negative or positive?
17 August 2022 7,928 5 View
We never had an issue with our MDLs not showing up until recently and I don't know why. Maybe the column is shot? Also the baseline seems to be lifting towards the end of sulfate and have no idea...
16 August 2022 9,916 2 View
Dear Sir and Madam, I have read many research articles that synthesize NPs via green methods, but none of those procedures didn't mention the addition of surfactant. However,few of my experiments...
16 August 2022 763 1 View
regarding the Characterization of PNP, what is the effective way to do FESEM ? running FESEM for (powder or dispersed(solution)) sample give a good result? regards
08 August 2022 8,319 5 View
Dear All, I am working with E. coli and want to determine its fatty acid content using GC-MS. I have converted fatty acids into FAMEs by chloroform/methanol method and have my samples in...
03 August 2022 5,088 3 View
This required for electrospinning of PCS at room temperature.
02 August 2022 8,122 1 View
Hello? I'm analyzing the photolysis products of organic molecule through LC-MS. Recently, we were able to obtain stable LC-MS results and are currently trying to obtain quantitative...
01 August 2022 608 2 View
How to perform a reliable measurement of the OH radical in the Fenton reaction. I spend AOP in the water. It is necessary to measure the OH radical, but the standard method for converting...
28 July 2022 950 0 View
In kejldahl methode for nitrogen content i m getting blue color insted of green in distillation step when i m adding boric aid indicator why? Please suggest me some solution of this problem
05 July 2022 8,180 5 View
How do I calculate the organic loading in kg VS m-3? I'm examining the amount of methane produced from anaerobic digestion. This was carried out in 125 ml serum bottle with a working volume of...
01 July 2022 8,593 0 View