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Women’s top 5 turn-ons. 1. Romance 2. Commitment 3. Communications 4. Intimacy 5. Non-sexual touching (Allan & Barbara Pease)
14 September 2025 1,241 2 View
Hello everyone, I performed the manual MDA assay using 200 µL plasma, 500 µL sulfuric acid, and 200 µL phosphotungstic acid, followed by centrifugation. The pellet was then dissolved in 400 µL of...
14 September 2025 5,975 0 View
I have performed molecular docking of multiple ligands against a target receptor. I want to perform MD simulation of the protein ligands complex using CHARMM-GUI and NAMD2. How will I generate...
11 September 2025 8,856 3 View
What new insights does fractal geometry offer in modeling natural phenomena?
09 September 2025 4,576 1 View
Hello everyone, I am performing the MDA assay manually. In my protocol I use sulfuric acid, phosphotungstic acid (PTA), and thiobarbituric acid (TBA). I have three specific questions: 1. What are...
06 September 2025 1,771 1 View
I performed several Bradford assays to quantify proteins in human serum, but the absorbance of undiluted serum (with and without protease inhibitors) consistently showed negative values or values...
03 September 2025 2,688 3 View
How can it be measured separately for each component from Ag NPs and asparaginase enzyme or as total absorbance? How was background scattering handled?
28 August 2025 3,013 2 View
I am simulating an all-solid photonic crystal fiber, and its confinement loss is very low (10^-6). Can I trust this result? I infer that the absorption loss has not been calculated. How can I...
21 August 2025 8,602 0 View
I am using a CW laser source of wavelength 532 nm. I have calculated the incident power density of the excitation source. Now I need to calculate the induced carrier density in my material (i.e.,...
21 August 2025 5,288 1 View
Actually, I want to know that how I could connect the Environment impact Assessment and Mega Infra Structure Project in any research work.
20 August 2025 6,298 1 View
I am performing an ELISA to detect specific antibodies. The kit includes a positive and negative controls, as well as the cut-off controls. However, the protocol indicate "measure blank on air" in...
20 August 2025 9,752 1 View
I'm studying the water column and have found the mixed layer, where heat and matter transfer occur. Its definition is different from the thermocline, but they appear to be located at nearly the...
19 August 2025 4,341 0 View
Hi Dear Hi, dear. I have attached a figure. I want to determine the band gap energy from the Tauc plot. I have drawn tow lines on the figure, as you can see. Could you please tell me which line...
11 August 2025 1,733 3 View
Hi everyone, I have a question regarding an experiment. I would like to slow down the growth of fibroblasts (JNHF) in a co-culture setting, so that the melanoma cells cultured alongside them have...
05 August 2025 6,282 3 View
What are the limitations of using persistent homology in data analysis?
05 August 2025 2,996 1 View
I have synthesized MXenes and evaluated its electrocatalytic performance for hydrogen evolution reaction. The MXene is made into an ink using ethanol and Nafion and is drop casted on glassy carbon...
01 August 2025 1,934 2 View
i am using gldas as initial data and era5 as forcing data and remapped era data according to gldas. and i used gldas vtable in hrldas createforcing.exe . i used dynamic veg option 1 but having an...
31 July 2025 4,723 0 View
In the bottom-up growth mode, how to understand that spherical assembled nanoparticle catalysts exhibit superior stability compared to conventional supported nanoparticles?
25 July 2025 9,104 1 View
I am calculating the lattice mismatch of AlN (100) thin films grown on Si, Mo, and Ti substrates using the measured d-spacing values obtained from HRTEM/SAED. Is it valid to use d-spacing (rather...
21 July 2025 7,948 6 View
I am working on Gateway cloning and using primers that contain attB sites. The primers are 49–50 nucleotides long, and their melting temperatures (Tm) are approximately between 70–76 °C. However,...
18 July 2025 5,615 1 View
can anyone please provide me the namelist.input for hrldas because the way i am naming my forcing my data in namelist provide the same output (initial one) that means my hrldas is running on...
17 July 2025 4,729 2 View
Hi, I'm currently working on extracting protein from iPSC-derived cardiomyocytes for Western blot analysis. For each experiment, I'm using approximately 8 × 10⁵ cells. I’m wondering whether this...
15 July 2025 8,070 3 View
I have a question about analyzing the antibiotic amoxicillin with spectrophotometry: To determine the absorbance of amoxicillin, can it be dissolved in distilled water and then examined using a...
14 July 2025 1,637 0 View
Hello everyone, Recently I was running trastuzumab on native (tris glycine) gel from bio-rad in tris glycine running buffer (No SDS) pH 8.3. However, I didn't get any band on the gel. I checked...
07 July 2025 5,657 2 View