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Questions related to Organic
Introduce The discussion around Environmental, Social and Governance (ESG) criteria and corporate sustainability has gained significant momentum in recent years, largely driven by societal...
16 December 2023 8,874 1 View
I did a factor analysis and identified two factors (i.e. intrinsic satisfaction and extrinsic satisfaction). Then on Mplus I did a regression of the outcome variable on factor scores: overall...
15 December 2023 6,673 7 View
I need to dissolve sulphate to purify the nanozyme synthesized with magnesium sulphate
12 December 2023 9,685 6 View
I observed bond breaking during the optimization of negatively charged molecules using various computational methods. This issue was persistent across different levels of theory. I am seeking...
10 December 2023 2,456 3 View
What microorganisms forms that increases soil fertility and beneficial microorganisms improve soil fertility?
09 December 2023 3,544 30 View
I am using the FRET pair to look at protein conformational dynamics, but while calculating the cross-correlation curve between donor and acceptor, I am observing asymmetry in the cross-correlation...
08 December 2023 7,883 0 View
I am trying to use BES, 2-(bis(2-hydroxyethyl)amino)ethane sulfonic acid, as one of the components for a polyurethane synthesis, but it does not dissolve in most solvents; like DMF, DMAc, and ACN....
06 December 2023 2,165 2 View
If the response is yes, how about the detection of ion channel proteins in homogenates by Western blotting?
06 December 2023 5,108 0 View
I am relatively new to conducting Western blots. I employed the alkaline lysis method to extract whole proteins from yeast. Subsequently, I introduced the Opti Protein Marker (ABM, CAT log NO:...
06 December 2023 1,436 5 View
Where is the electric field strongest and relationship between the density of the equipotential lines and the strength of the electric field?
06 December 2023 4,330 0 View
Hello ResearchGate Community, I am searching for a water-soluble dye that can withstand a prolonged period (one month) at 85°C and 5000 psi. It's crucial that the dye remains stable and retains...
05 December 2023 1,614 3 View
Hey guys, I tried electrospinning PLA nanofibers on aluminium foil. I was able to achieve thicker fibers by increasing the viscosity of the solution. However, the fibers stick to the aluminium...
04 December 2023 3,439 5 View
I am working in validation and qualification of new HPLC method. Your insights and feedback are greatly appreciated.
03 December 2023 8,535 4 View
internal error in GENERATE_KPOINTS_TRANS: number of G-vector changed in star 113304 113306 How to deal with this situation? I see the official website of vasp suggests to cancel...
02 December 2023 9,620 0 View
Hi all, I was doing protein purification and after that I had to add 2 uL DTT (1mM), but instead, I added 1000 uL DTT (1mM) to my protein solution. I would like to ask you, if my mistake has any...
01 December 2023 1,138 6 View
My samples salinity is less or more 300 g/L. I would like to determine especially the concentration of trace elements which should be around 30 to 90 ppm. The issue is with dilution the initial...
29 November 2023 1,606 6 View
Upon disruption of the disulfide bonds of the protein by a reducing agent, will a phase transition occur? Is the outcome of this phase transition related to the quantity of disulfide bonds present...
25 November 2023 3,480 0 View
What are the recommended fluorophores for in vivo tumor imaging using the IVIS system? I am particularly interested in options compatible with IVIS imaging and would like information on the limit...
20 November 2023 4,023 0 View
If we have a leaching solution containing a mixture of heavy metals in a wide range of concentrations (for example, we have some heavy metals in solution in 10 - 100 mg/L concentration range while...
20 November 2023 8,527 4 View
Hi everyone, I'm currently working with a plasmid called pBI101, I isolated this plasmid from a plate then I did the extraction with mini prep protocol and cut it with SmaI at 25°C incubation...
18 November 2023 6,997 3 View
Thanks
18 November 2023 9,294 0 View
I have four DNA fragments for Gibson Assembly; two of the fragments are 5kb with a concentration of 0.011pmole/ul and 0.034pmole/ul, and the other two fragments are 0.5kb (0.163pmole/ul) and 0.6kb...
17 November 2023 5,887 0 View
What actually the reason of change of anode material, why due to charging anode material act again as electron reservoir so that Li ion or Na ion can take it. It would be helpful if I got the...
17 November 2023 1,179 1 View
looking at a electricity grid that has renewable such as wind, i want to model the effects a synchronised condenser has on the rate of change when there is either or sudden drop or surge in...
16 November 2023 4,921 3 View