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Hi everyone, I am going to design a template for in vitro transcription. I add the T7 promoter sequence (taatacgactcactatag) before the target template sequence and insert two other Gs after the...
21 April 2022 9,561 0 View
I have tried three methods : -column chromatography (using basic alumina, did not work very well) -dialysis using a spectra labs membrane. Effective but leaving the PAM in water for 2-3 days...
15 April 2022 9,078 0 View
In a solid(powder) sample, it can be done using XRD or any other characterization method. Could you please suggest a method to find the SiO2 amount dissolved in a liquid sample?
07 April 2022 332 0 View
In the case of solution state NMR excited nuclei relax back to ground state either by spin-spin or spin-lattice relaxation. The influence of adjacent nuclei in signal intensity is seen in hydrogen...
22 March 2022 8,607 0 View
I am using Nafion to cover my electrodes used to make biofuel cells, in order to block chloride ions from reaching the electrode. Unfortunately, at a physiological concentration of chlorides,...
18 March 2022 7,816 0 View
I'm looking for a reaction condition that could selectively activate the aliphatic ester within my compound, to convert it into an amide with a primary amine. However, there is also a lactone...
14 March 2022 8,592 3 View
I am trying to have a thin monolayer of gold nanoparticles on acrylic surface, but I don't find a suitable option for doing that. I tried thermal vapor deposition but that makes a continuous film,...
08 March 2022 2,942 9 View
Hello! I have a co-amorphous system with API-Tryptophan and API-Phenyalalnine obtained milled. Amino acids could not be transformed into an amorphous form via ball milling, so I cannot get the...
04 March 2022 5,704 3 View
Hi everyone, I am trying to find out the size of cyanuric acid molecule. Does anyone know any technique/models/publications which can help me to know the size of this particular molecule. Any...
03 March 2022 5,361 0 View
Greetings and Regards I calculated the bacteriorhodopsin concentration in Halobacterium salinarum as described by Shand and Betlach. But I have a question: why does bacteriorhodopsin lose its...
03 March 2022 2,877 8 View
1. What is the best way to prepare samples for the Raman analysis, the sample in question is a colloidal solution. So far I have come across two different methods to do so, (a) To drop cast a...
01 March 2022 6,086 4 View
Required for PhD candidate for interview point
25 February 2022 8,536 7 View
I am in the process of writing my thesis in peptide synthesis. My project deals with CGRP analogues synthesized by spps. In my synthesis of the CGRP analogues, I use following pseudoproline...
22 February 2022 3,594 4 View
I would like to know the culture collections or other private institutions who could provide yeast strains for protein production possessing strong promoters, selection markers and possessing...
17 February 2022 9,748 6 View
I did a nonlinear static analysis (Pushover) on a RC bracing moment frames with 2 spans and 3 levels (2D model) using SeismoStruct software. Beam-Column Element types used is ==> Inelastic...
16 February 2022 3,944 4 View
hello, I am working with dynamic time-history analysis using seismostruct softwar, in order to calculate the pushover of the structure under earthquake. Although, I didn't found data about seismic...
16 February 2022 9,818 5 View
Reaction of I with palladium acetate, 1,3-bis(dicyclohexylphosphino)propane (DCPP), K2CO3, water and NMP under 30 psi of carbon monoxide at 120 C gives II. Please explain the mechanism for the...
15 February 2022 1,581 3 View
Greetings everyone, I recently encountered a really weired situation and couldnt really figure out why. Im doing a reaction involving using epoxide to neutralize unwanted acidity of the original...
15 February 2022 9,646 0 View
Hello everyone I am going to fabricate a hydrogel using PEG-SH via a thiol-en reaction. It is well known that PEG-Thiol is prone to oxidation, and it has been suggested in the literature that it...
02 February 2022 7,184 2 View
Generally, In thermoplastic polyurethane elastomer (TPU), polydiol chains, known as soft segments and diisocyanate chains, called hard segments are arranged in a certain ratio. This hard and soft...
01 February 2022 1,508 1 View
I want to know what are the controlling factors that differentiate whether this ionic pairs are going to form ionic liquids or not, any help is highly appreciated.
31 January 2022 6,618 10 View
I want to make 1.6 and 2 micrograms per 20 microliters of capsaicin. I did it with 80 and 100 microgram Capsaicin respectively in 1ml DPBS solution. But it is difficult to measure 80 or 100...
28 January 2022 759 2 View
I tried different chiral catalysts but didn't work.. many thanks..
27 January 2022 4,175 13 View
I want to know the best technique that provides exact and reliable results on the chemical composition of a plastic product (geosynthetic product) with the concentration of each compound what is...
24 January 2022 1,917 5 View