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Questions related to Organic
What is the entropy of a pure crystal and why entropy of a perfectly crystalline substance is less than that of its imperfect crystals?
03 June 2023 4,560 1 View
Hi, I have been using HighScribe T7 polymerase kit for in vitro transcription from NEB, but the protocol gives a 20uL reaction with 1ug DNA template, which is not enough for my downstream...
31 May 2023 1,169 3 View
Is it possible to establish baseline separation for two polymorphic proteins roughly having 50 and 54 kDa MWs by using any polymeric-based SEC column at analytical scale? Any product or shared...
31 May 2023 1,742 2 View
Has someone quantified the same pool of samples by both methods? How well do they correlate? Thanks
30 May 2023 8,843 0 View
I'm trying to obtain xylose crystals from the aqueous phase of an acid hydrolysis-derived process. After evaporating the water, we performed an ethanol extraction in cold. It was supposed to yield...
19 May 2023 6,265 2 View
Using LaGrange formulation we write the work done by non conservative forces in terms of force or moments as it is? or taking partial derivatives?
17 May 2023 6,684 0 View
I've been optimizing a Western protocol for phosphorylated proteins and have had issues with my red channel. Proteins in my green channel would not show up in my red channel. I would also have to...
16 May 2023 8,959 0 View
Hi everyone. I'm working on DFT studies for conjugated polymers. Currently, I'm using 6-31G(d,p) basis set for calculating HOMO-LUMO. Would 6-31G(d,p) is good enough basis set for conjugated...
15 May 2023 5,532 1 View
Respiridone contains mainly 2 forms which will be A and B.
11 May 2023 2,204 0 View
In my sample , the red and blue shift occurred, what is the explanation for that?
10 May 2023 2,559 1 View
We performed qPCR analysis using SybrGreen dye, where the template was gDNA, on the Applied Biosystems 7500 Fast Real-Time PCR System (software ver 2.3). The reaction conditions were optimized...
28 April 2023 6,186 3 View
I am trying to do GST pull down with my experimental proteins and GST as my negative control. however, my negative control (GST) is interacting very strongly to my target protein and even lowering...
26 April 2023 8,227 0 View
Phenolphthalein changes colorless at less than Ph 8.3 and light red at 8.3 to 10.0. I wonder if the phenolphthalein solution is colorless or red at acidity above Ph10.0.??
25 April 2023 5,501 1 View
Hi all. I am facing trouble with my alumina (neutral activated Grade II), with which I used to separate marine fuel components on a chromatography column packed with alumina gel made of alumina...
24 April 2023 8,629 0 View
Hi everyone I am facing the attached problem while installing ligplot program on windows 11. I would be very happy if you could help.
19 April 2023 622 0 View
In MD simulation, I see that the peptide loses its disulfide bond as a result of high temperature application. I can't see the disulfide link in PyMOL, but I can see a link with the SG code in the...
16 April 2023 3,589 1 View
Actually I am interested to know Fenton reaction with surfactants solution and its priorities in the lab? so thanks
16 April 2023 9,278 0 View
I was trying to calculate the ESP in the ground and the 1st excited state (Gaussian 16). ground state: POP=chelpg IOp(6/33=2) excited state: TD=(Read,Root=1) density=current POP=chelpg...
28 March 2023 6,900 4 View
Hi, I have a QE HFX and last winter the protein hits dropped and the field engineer found all evaluations on the MS passing but hits did not improve until the electronics board was replaced. RF...
27 March 2023 1,236 0 View
This is question is based on Green synthesis of plant materials using Silver Nitrate
26 March 2023 6,581 2 View
I find it can be detected by CV technique but these authors don't give details, so please help me if you know, thanks.
20 March 2023 7,549 5 View
I think yes, what do you think? Please share your own ideas.
18 March 2023 3,383 5 View
I need to dissolve 4′-Bromo-3′-nitroacetophenone in methanol or ethanol for use in Chalcone synthesis by Claisen-Schimdt condenstaion reaction. Can anyone guide if it needs temperature too? I have...
14 March 2023 424 3 View
When Rose Bengal is added to fentanyl; it should show a color change from red to purple due to the new complex made by binding O- of RB and N of FTN (depicted in a black arrow). But it does not...
14 March 2023 4,350 0 View