About Spectrophotometric determination of amoxicillin amount?

14 July 2025 0 2K Report

I have a question about analyzing the antibiotic amoxicillin with spectrophotometry: To determine the absorbance of amoxicillin, can it be dissolved in distilled water and then examined using a spectrophotometer and a calibration curve drawn for it? I did this and my analyte had an absorbance at 383 nm. According to the literature, Why we should use NaOH for its hydrolysis when it completely dissolved in water... I have the same question about Amoxicillin+clavulanic acid?

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