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Hi, Could anyone please help me understand why a shadow appears beneath my protein band in a western blot? Also, any suggestions for a solution would be greatly appreciated. I have attached an...
29 January 2025 915 4 View
Hi, I recently began working with THP-1 cells from ATCC and have followed the manufacturer's protocol, incorporating 2-mercaptoethanol and 10% heat-inactivated FBS. I am using RPMI from Thermo...
28 January 2025 5,191 2 View
I am working on photocatalytic water splitting from a suspended catalyst. I can measure the total volume of gas evolved from the water displacement method. But by this method, I cannot store my...
27 January 2025 2,738 1 View
can any one suggest how to draw axial ratio vs frequency plot in CST
25 January 2025 3,166 0 View
I want to make 30 ml.
24 January 2025 3,990 2 View
Dear everyone, I am performing gas phase catalytic reactions, where my products are detected with a mass spectrometer. Regarding the data evaluation I have the following questions: 1. Do I need...
22 January 2025 909 0 View
I am working on a gene that has 14 variants, and I am specifically interested in knocking down one particular variant. After sequence alignment, I discovered that the variant I want to target has...
21 January 2025 9,606 0 View
We are on the edge of change that affecting all humanity.
20 January 2025 2,351 2 View
When I try to install this software, it will appear like this. Does anyone have the solution for this error? This I attached with the list of files
18 January 2025 2,073 1 View
I tried to cyclise this (E)-2,3-di(thiophen-2-yl)acrylic acid to form cyclic ketone through Friedel Crafts reaction. The condition was forming the acid chloride using oxalyl chloride in anhydrous...
13 January 2025 8,204 6 View
The South African(SA) government is planning to shift the countries energy systems away from coal and towards renewable energy sources. The government has come up with a program called Just Energy...
09 January 2025 3,345 2 View
I want to conjugate DNP (2,4-dinitrophenyl, molecular weight: 394 Da) to an IgG antibody (molecular weight: 150 kDa) in a 1:5 molar ratio (1 antibody to 5 DNP molecules). I would like to know: How...
07 January 2025 6,326 0 View
During winter, we face significant challenges such as cold walls and condensation, even with 10 cm insulation. To address these issues, what simulation techniques can be employed to optimize heat...
06 January 2025 8,454 3 View
This research seeks to explore the intricate relationship between immune cell metabolism and the pathogenesis of autoimmune diseases. While immune responses are typically studied through the lens...
02 January 2025 2,420 2 View
Which software is suitable for modeling anisotropic stress behavior of different rock variants during thermal heating? COMSOL/ABAQUS/ANSYS If COMSOL, then which module should be proceeded with for...
01 January 2025 8,580 3 View
i have tried both time and frequency solver an d i have got two different results. s_{11} and far-field are very different getting from frequency and time solver I saw videos on YouTube that used...
29 December 2024 1,668 2 View
Hello, I am trying to determine rate coefficients for a reaction using Phase Space Theory (PST), but I need clarity on how to apply this method step-by-step. I would appreciate detailed insights...
24 December 2024 1,826 0 View
Fiber optic sensors play a transformative role in structural health monitoring (SHM) because of their advanced and meaningful features tailored to complex infrastructure needs. These sensors offer...
24 December 2024 4,407 2 View
organic liquid fertilizer production project report?
19 December 2024 3,819 2 View
- What is its importance in ionic exchange of sandy soil? - What is its role as an organic material in capacity exchange cation of sandy soil? - Its importance in the availability of elements in...
17 December 2024 8,933 3 View
What is the effect Plasmonic metasurfaces with quality factors in the visible regime ?
12 December 2024 1,532 1 View
I need to perform MIC 90 and MIC 50 of a bacteria. I have found papers where they are performing the experiments with 10 or 5 different strains of that bacteria which I need to know the MIC 90/MIC...
12 December 2024 1,150 1 View
i want to simulate a superconducting microstrip line using harmonic balance method in a 3D-FEM app like comsol . if anybody know about it , let me know .if you have any file that can help me...
11 December 2024 2,164 1 View
Hi everyone, I am trying to launch some anharmonic calculations on methane and its tritium derivatives, but I am facing some problem: the calculated anharmonic frequency are pretty far from the...
11 December 2024 2,240 0 View