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Questions related to Organic
In industrial PECVD system, anyone face burn-mark issues on final wafer?
16 November 2023 6,942 1 View
1. One of the main principles of supercapacitors is electric double layer building. This is the principle that a double ion layer is formed when the electrolyte is close to the electrode surface....
15 November 2023 5,574 1 View
Electrospinning is a voltage-driven, fabrication process governed by a specific electrohydrodynamic phenomenon where small fibers are yielded from a polymer solution. how can I use in periodontal...
14 November 2023 7,246 1 View
I expressed the protein(46kDa, PI=9.04) with His tag in E.coli and purified it with cobalt beads. However, when I was using Amicon Ultra 15 mL Centrifugal Filters to concentrate elution flowed...
14 November 2023 2,942 3 View
Will the TrxA tag in the E. coli prokaryotic expression vector pET32a cause non-specific binding in WB or ELISA experiments? I used a fusion protein with TrxA to coat an elisa plate to test...
14 November 2023 8,601 1 View
How long (approximately) would it take to learn DFT calculations from scratch so as to be able to obtain the first reasonable results? The aim would be to design materials with intended properties...
13 November 2023 8,064 4 View
According to these results, Is regression analysis significant? Analysis of Variance - SS = 1904905 - DF = 8 - MS = 238113 - F (DFn, DFd)= F (8, 17) = 1.353 - P value P=0.2843 Goodness of...
13 November 2023 2,504 11 View
Hello all, I am currently trying to purify a fragment of the large surface protein of HBV however during purification I notice that a white precipitate form on the Ni-NTA column. (At pH 8.0; pI of...
08 November 2023 7,145 4 View
Hello, I have been reviewing several articles on DNA methylation quantification for aging studies. I don't want to do a complete genome DNA methylation quantification, since it is quite costly,...
03 November 2023 3,014 2 View
I want to know the composition of high volatile compound present in soil. So, which technique will give me the right information of all organic elements present in that high volatile compound? As,...
31 October 2023 9,044 1 View
Can anyone suggest me how to calculate charge transfer integral of organic semiconductors having crystal data available in CCDC, using material studio or any other free softwares
30 October 2023 3,755 1 View
I´ve been trying for the past weeks to create a plasmid through Gibson Assembly. My construct is pretty big (28kb). I am fragmenting a plasmid into 5 fragments and adding one extra fragment of 2kb...
30 October 2023 9,448 3 View
Greetings Everyone, I need some suppliers which can provide Protective Microwave Transparent Paint for Antenna PCB. Kindly let me know the details of the suppliers.
30 October 2023 2,592 1 View
It has to be something within these topics and with only free of charge publicly available datasets/sources. Thank you for any tips/hints..
28 October 2023 8,816 4 View
I'm working on my thesis about formulating an adhesive for sticky traps and am currently having trouble finding a suitable procedure with exact measurement that i can revise. Any recommendations?...
28 October 2023 8,781 3 View
hello everyone, i hope i can get help on this. so i knockdown a gene using RNAi and i see its knockdown phenotype on the plate, however when i extract the RNA and reach qPCR i see upregulation in...
26 October 2023 660 1 View
Hi. I am interested in the following: having a high resolution spectrum by NMR (e.g. a 400 MHz), I want to modify it digitally to see how the same spectrum would look like in low resolution (e.g....
24 October 2023 9,893 8 View
I recently synthesized several ChCl: xylitol-based NADES without water, which were immediately transferred to sealed glass vials until further use. Some of the NADES remained transparent in the...
24 October 2023 6,581 0 View
Hi all, I work with the MC38 colorectal cancer model and the goal is to dissociate tumor infiltrating immune cells with minimal loss. There is also massive tumor cell death if a therapy is...
24 October 2023 3,743 1 View
I am setting up Cyclic voltammetry with Ag/AgCl electrode, glassy carbon electrode and Pt wire elctrode. But, I don't know that how to apply internal standard. I know that to do CV in an organic...
23 October 2023 3,908 2 View
In using Opt TD=(nstates=n) in Gaussian, I get many data points for excited state energies of each state, i.e. the results for the TD calculation are repeated after each optimization. Should I...
20 October 2023 4,168 4 View
I am doing research on internal soil erosion mechanism. ICOLD Bulletins 164 is an important reference material, but I am not an ICOLD member and cannot get the report. I hope you can help me get...
19 October 2023 7,118 1 View
Hello! I'm trying to perform site-directed mutagenesis, similar to the QuickChange II protocol, on a plasmid of approximately 9 kB using Q5® High-Fidelity DNA Polymerase. I've attempted various...
18 October 2023 3,571 3 View
When I conduce an esterification reaction using oleic acid P.A, occurs a variation in color of final product generated (liquid). Is this color due occurence of some concurrent reactions generating...
17 October 2023 8,459 1 View