Does anyone know for a commercially available defined media for S.aureus?

More Karen Butina's questions See All

Way to oxidize flavins and related molecules?

I would like to oxidize flavins and if possible also quinones in bacterial supernatant. Is there an enzyme one could use for that? Or any other "oxidant"? I use M9 minimal medium. Thank you so...

10 November 2017 445 3 View

Can one fix bacteria with isopropyl alcohol?

I would like to fix S.aureus on plastic surfaces and I cannot use EtOH or acetone since they dissolve my plastics and I would just like to try another method then formaldehyde. Than you so...

09 October 2017 4,714 3 View

Mapping PEDOT:PSS conductivity with PeakForce TUNA?

I have spin coated films of PEDOT:PSS on glass (and gold) and would like to map their conductivity The films are about 150nm thick. I have tried using PeakForce TUNA (Dimension Icon, Bruker) but...

08 September 2017 6,686 2 View

Sequential spin coating of the same material to increase film thickness?

I am working with PEDOT:PSS thin films that I routinely produce by spin coating on play microscope slides. For various measurements I would need to have films of different...

06 July 2017 433 9 View

Is there a transposon library of S.aureus 8325-4 one can obtain?

I am looking for transposon library in S.aureus 8325-4 strain.

06 July 2017 2,674 6 View

Can bacteria be cryofixed?

I am using a conformational dye that bonds to some structures on bacterial membranes and formaldehyde fixation does not really work for me. Thanks!

04 May 2017 4,576 5 View

NADH fluorescence in filtrates of bacterial cultures increases upon heating??

Upon filtering (0.2um) or ultrafiltering (3kDa) an exponential culture of Salmonella enteritidis in M9 I can measure fluorescence at the wavelengths expected for NADH. However, if I heat these...

10 November 2016 2,033 5 View

Why am I unable to blot a recombinant protein from mouse liver ?

We have used hydrodynamic injection (http://www.mjhid.org/article/view/418/620) to deliver DNA to liver where we have shown with microscopy and IHC that the protein is present. We have also used...

01 February 2015 5,201 12 View

Can anyone suggest other tags for purifying proteins under denaturing conditions other than Histidine-Tagged?

We would like to find an option to purify proteins under denaturing conditions (= 6M guanidinium hydrochloride) that is not his column (or similar, cobalt etc). Any ideas? Thanks a lot!

01 February 2015 3,606 10 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View

How does social media influence people's dental habits and interest in aesthetic dental treatments?

Hello, Dear friends, I am surveying as part of my thesis research and would greatly appreciate your participation. The study investigates how social media influences dental health behaviors...

01 August 2024 9,349 2 View

The use of data from PubChem for commercial purposes?

Hi, I'm curious to know if data on chemical compounds from PubChem, such as water solubility properties, can be used to train a machine learning model for commercial purposes. Will this infringe...

30 July 2024 8,707 1 View

What is the best method to detect the chemical composition of pollen ?

I have multiple small samples of grass pollen with anthers. I want to find a method to identify concentrations of proteins, lipids, and other various elements for these samples. any suggestions?

28 July 2024 5,331 1 View

How to maintain the drug concentration in cell culture study for long term?

I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...

24 July 2024 8,696 0 View

How can I identify popular hashtags on a social media platform from three years ago?

I'm looking into climate change fake news (specifically ice melt fake news) on TikTok and YouTube, and I'm going to be gathering relevant social media posts from around COP26. Any ideas on how I...

23 July 2024 6,737 2 View

Could international experts in media and journalism, AI, communications and international communication share this conference link?

"Integration of Mass Media and Communication into the System of International Relations: Place, Role, Actuality and Prospects: Modern Media Issues 2024" Website: https://immcir.alnoor.edu.iq/

23 July 2024 3,139 6 View

I'm conducting a study on Research Commercialization in Pakistani HEIs and would appreciate your participation in a brief survey?

Dear Participants- Faculty and Researchers from Pakistani Higher Education Institutions, Thank you for participating in research study that aims to 'Analyze Individual and Institutional Factors...

18 July 2024 7,024 1 View

How to prepare bacterial conditioned media to study the effect of bacterial secretome?

I would like to investigate the effect of bacterial secretome on cancer cells. How do you prepare conditioned media from bacteria?? conditions- time points - media I am looking for the best way...

17 July 2024 3,785 9 View