Way to oxidize flavins and related molecules?

I would like to oxidize flavins and if possible also quinones in bacterial supernatant. Is there an enzyme one could use for that? Or any other "oxidant"? I use M9 minimal medium. Thank you so...

10 November 2017 342 3 View

Can one fix bacteria with isopropyl alcohol?

I would like to fix S.aureus on plastic surfaces and I cannot use EtOH or acetone since they dissolve my plastics and I would just like to try another method then formaldehyde. Than you so...

09 October 2017 4,577 3 View

Mapping PEDOT:PSS conductivity with PeakForce TUNA?

I have spin coated films of PEDOT:PSS on glass (and gold) and would like to map their conductivity The films are about 150nm thick. I have tried using PeakForce TUNA (Dimension Icon, Bruker) but...

08 September 2017 6,544 2 View

Sequential spin coating of the same material to increase film thickness?

I am working with PEDOT:PSS thin films that I routinely produce by spin coating on play microscope slides. For various measurements I would need to have films of different...

06 July 2017 301 9 View

Is there a transposon library of S.aureus 8325-4 one can obtain?

I am looking for transposon library in S.aureus 8325-4 strain.

06 July 2017 2,565 6 View

Can bacteria be cryofixed?

I am using a conformational dye that bonds to some structures on bacterial membranes and formaldehyde fixation does not really work for me. Thanks!

04 May 2017 4,458 5 View

NADH fluorescence in filtrates of bacterial cultures increases upon heating??

Upon filtering (0.2um) or ultrafiltering (3kDa) an exponential culture of Salmonella enteritidis in M9 I can measure fluorescence at the wavelengths expected for NADH. However, if I heat these...

10 November 2016 1,851 5 View

Why am I unable to blot a recombinant protein from mouse liver ?

We have used hydrodynamic injection (http://www.mjhid.org/article/view/418/620) to deliver DNA to liver where we have shown with microscopy and IHC that the protein is present. We have also used...

01 February 2015 5,041 12 View

Can anyone suggest other tags for purifying proteins under denaturing conditions other than Histidine-Tagged?

We would like to find an option to purify proteins under denaturing conditions (= 6M guanidinium hydrochloride) that is not his column (or similar, cobalt etc). Any ideas? Thanks a lot!

01 February 2015 3,486 10 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

Fictitious Boundary Meshing Method (FBM) in Commercial FEA software?

Is it possible to implement FBM in a commercial FEA software package, such as FEMLAB, COMSOL, or open source software like Moose?

22 February 2021 2,968 1 View

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22 February 2021 9,932 11 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Any suggestions or recommendations on a good publishing house based on the African for academic books in the field of Communication and media studies?

I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...

19 February 2021 2,482 2 View

Are there papers on?

I'm looking for a papers concerning either Christianity or neopagan religions and social media. What role does social media play in the spread of these religious movements? Any suggestions on the...

15 February 2021 655 4 View

A film coverage that allows for transmittance of wavelengths on the infrared scale?

I've landed on needing some type of highly optically transparent film or cover material that prevents evaporation of culture media; I am not sure which subfield of materials science would be able...

08 February 2021 5,760 1 View

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Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View