What are the activation functions to be used for a regression based Artificial Neural Network?

I am designing a Neural Network with two hidden layers for a regression problem in Python. While all inputs are positive, there are supposed to be negative values in the output. The model was...

09 July 2020 6,308 3 View

whta will happen if PCR Program is stopeed in between?

I accidentally set the initial denaturation at 95 degress for 1 min, but It should have been for 5 mins. I realised it after I started the program while it had reached denaturation step at 95...

27 August 2019 9,929 5 View

Why am I not getting DNA by alkaline lysis method?

I isolated plasmid DNA by alkaline lysis method.I am getting a very weird graph in nanodrop. No peak was seen at 260 nm.Can anyone please tell what is the problem?

18 August 2019 9,590 3 View

Python in material science ??

Use of python scripts in making supercell construction of materials (for DFT calculation)

26 May 2019 8,718 1 View

Why Shannon entropy of EEG gives negative value in Matlab ?

With EEG signals, wavelet (Shannon) entropy gave negative values. Can anyone explain why?

05 April 2019 8,209 5 View

Does ethanol, 1-butanol, acetone, methyl acetate, THF, and gamma valerolactone falls under the solvents from natural origin?

Please refer me some solvent names from herbal origin, having high volatility

25 November 2018 4,032 3 View

Is mass a scalar quantity or a tensor quantity?

We had an inconclusive debate today in class on whether mass is a scalar quantity or not. On checking the Internet I found that by using mass- energy equivalence, relativistic mass is found to be...

24 July 2017 5,179 17 View

Is it necessary to have connected objectives in a Ph.D thesis?

Is it always necessary to write a thesis where one objective of a Ph.D topic will lead to another objective and so on? What about the acceptability of a thesis with a broader topic and three...

09 January 2014 6,067 35 View

How to convert the DN values to reflectance?

The metadata of Landsat TM and ETM+ contains the DN values. How we can convert these DN to reflectance for these satellite in ERDAS imagine? Is the conversion satellite specific or feature specific?

30 September 2013 449 7 View

How to calculate rainfall intensity?

Can we calculate rainfall intensity with daily rainfall data? I have a set of 30 years daily precipitation data for two stations, will it be possible to create frequency-intensity-duration curve...

02 September 2013 4,797 10 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

28 February 2021 274 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

No title

22 February 2021 9,932 11 View

Alternative to mediation analysis?

I am currently working on my dissertation and want to explore the role of competence as a mediator between the effect of colour of clothes on voting intention (focus on Western countries)....

21 February 2021 8,609 1 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View