C-18 chiral stationary phase are available for separation.

I Think you can use chiral columns from the merck millipore company like ChiraDex®, ChiraSep® or ChiraSpher®. Sigma aldrich or Waters chromatography also have a good repertoire of chiral stationary phases in different type of columns. Good luck.

Bouchaib,

Hello! Do you have the exact name of the compound? For instance, does it have a generic name? If so, I can research it for you.

Chances are, if it has a generic name and is a fairly well known compound, you will be able to find a pre-existing application for it.

On the other hand, it if it an unknown chiral compound, or one that has not been studied before... the only way to find a good HPLC column is by experimentation. In general, you will want to get four or five chiral columns and run column screening tests (because of the nature of chiral columns and chiral compounds).

.

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Paul

All by earlier commentators give good solutions. If you know what the relationship is denoted, we can adjust the column. Usually this is the C18 as suggested by Robin, but równiaż M. Farooq is right that there is no happy medium. However, much depends on the same die, which is researching and in certain situations you have to test yourself doswiadczelnie column type and which is associated with this manufacturer.

Which rasemic compounds do you want to separate? There are so many chiral column. DAICEL compnay sell many typ of chiral column: http://www.daicelchiral.com/en/chiralcolumns/normal.html

Chiral method development is an advanced technique best suited to chromatographer's with many years of practical HPLC experience. Your method development skills should be strong as should your understanding of the fundamentals of chromatography (as with all chromatography, understanding Kprime, T0, R and S are very important). The most important key is to first identify the correct column for the sample which will retain it with good peak shape (finding the correct mobile phase is the second step).

Chiral method development by HPLC and/or SFC requires the use of many different chiral columns and mobile phases to find a suitable separation.

If you would like to learn about practical Chiral HPLC (and SFC) screening methods for pharmaceutical drugs (for Racemates and Enantiomers), then I would suggest you consult the following articles :

"Chiral HPLC and SFC Column Screening Strategies for Method Development:" http://hplctips.blogspot.com/2011/06/chiral-hplc-and-sfc-column-screening.html

"CHIRAL HPLC / SFC METHOD DEVELOPMENT (Alcohols):" http://hplctips.blogspot.com/2011/04/chiral-hplc-sfc-method-development.html

"Some Stereochemistry Terms (Chirality)" http://hplctips.blogspot.com/2011/06/some-stereochemistry-terms-chirality.html

"Modern HPLC Method Development Tips (PART I):" http://hplctips.blogspot.com/2016/07/modern-hplc-method-development-tips.html

http://hplctips.blogspot.com/2011/06/chiral-hplc-and-sfc-column-screening.html

http://hplctips.blogspot.com/2011/04/chiral-hplc-sfc-method-development.html

http://hplctips.blogspot.com/2011/06/some-stereochemistry-terms-chirality.html

http://hplctips.blogspot.com/2016/07/modern-hplc-method-development-tips.html