For some unknown reasons, the software (Empower 2) doesn't provide mass distribution values for my samples. It integrates the chromatogram and detects all the peaks/retention times very well but my results table is just empty on these parameters (Mn, Mw, Mp, Mz, etc.). It gives me the raw measurements of peak height, area, and %area as well. Is there any way I can use these measurements to do my calculations?
The methodology was as below:
Molecular weight characterization of the polymers were determined using a GPC unit (Waters 2695) equipped with a RI detector (Waters 2414), and a PDA (Waters 2998). The samples were characterized by passage through a guard column, followed by two Styragel 5 μm, HR 4E 7.8 × 300 mm column in series. THF was used as the eluent at a flow rate of 1.2 mL·min−1. Narrow poly(styrene) standards (580-156000 Da) were used to calibrate the GPC.
The chromatogram was obtained at 230nm wavelength and some of my peaks are within the range of standards.
Any comments, suggestions?