For some unknown reasons, the software (Empower 2) doesn't provide mass distribution values for my samples. It integrates the chromatogram and detects all the peaks/retention times very well but my results table is just empty on these parameters (Mn, Mw, Mp, Mz, etc.). It gives me the raw measurements of peak height, area, and %area as well. Is there any way I can use these measurements to do my calculations? 

The methodology was as below:

Molecular weight characterization of the polymers were determined using a GPC unit (Waters 2695) equipped with a RI detector (Waters 2414), and a PDA (Waters 2998). The samples were characterized by passage through a guard column, followed by two Styragel 5 μm, HR 4E 7.8 × 300 mm column in series. THF was used as the eluent at a flow rate of 1.2 mL·min−1. Narrow poly(styrene) standards (580-156000 Da) were used to calibrate the GPC.

The chromatogram was obtained at 230nm wavelength and some of my peaks are within the range of standards.

Any comments, suggestions?

Similar questions and discussions