I found a protocol to do Normal Goat Serum, but I am not sure if it is correct. Is it needed to make a heat treatment? It is for using in IHQ as blocking serum. Can anybody help me, please?
Normal Horse Serum Blocking Solution:
2% horse serum (blocking)
1%BSA (stabilizer)
0.1% cold fish skin gelatin (blocking)
0.1% Triton X-100 (penetration enhancer)
0.05% Tween 20 (detergent and surface tension reducer)
0.05% sodium azide (preservative)
0.01M PBS, pH 7.2
Mix and store at 4 ºC.
We use a normal horse serum blocking solution from Vector labs for IHC - its not overly expensive. This works very well for us.
Just to clarify we don't use this neat we dilute 1/20 in TBS immediately prior to blocking.
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