Hello everyone. I am doing immunization. after doing ELISA, i left serum collected from mice at room temperature for more than 10 hrs. Is there any chance that it is contaminated or my Antibody (anti-Agr2) is degraded?
Serum does not contain free proteases but the only way to know the answer is to use your sera again. If they are not sterile, I think 10hrs at RT (23°C probably) is not a long time for bacterial or yeast growth. Good luck
Hi Sehar, I agree with Paola. Sera has more proteinase inhibitors than proteinases.
If you have microbial contamination of your sera, it should be obvious by being cloudy.
Dear
Your sample could be degrade after long storage due to microbial contamination or instability of some amino-acids. Proline-containing peptides are known to be among the least stable peptides since they undergo rapid degradation in serum samples.
To obtain a stable serum, uncentrifuged clotted blood should be stored at 4 °C and processed within 6 h.
Please finf more information in the following publication: Stability of Endogenous and Added RNA in Blood Specimens, Serum, and Plasma. Nancy B.Y. Tsui, Enders K.O. Ng, Y.M. Dennis Lo. Clinical Chemistry Oct 2002, 48 (10) 1647-1653.
Hoping to be helpful
Marius
- Badges
- Science topic
- Similar topics
- Medicine
- Disease
- Infectious Diseases
- Immunization