I collect splenic cells from C57/BL6 mice at day 7 post immunization with OVA protein and CFA, then incubate the sorted CD8 T cells from splenocytes with OVA257-264 pulsed EL4 target cells for several hours. I wonder whether OVA immunization could give rise to CD8 T cell response and generation of CTLs? Thanks!
Hi Lianh Liu,
Actually it does.
we use OT-1 TCR Transgenic mice for the same purpose.
OT-2 TCR Tg mice are used for CD4+ T cell study and you do not need to immunize them as they are genetically engineered to constitutively express TCR that recognizes OVA MHCI or MHCII restricted antigens.
The best thing is u can do it straightaway without any immunization i.e. no tedious and prolonged immunizations and one day experiment.
Best of Luck!!!
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