https://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/5/s6322dat.pdf

Has anyone prepared a vial of sigma adjuvant systems (Ribi's) when they either aren't using the whole vial in one go or need to 1 vial for different antigens? The product insert says you should warm the vial to 40-45 degrees before adding 2ml of an antigen-saline solution. If you are only reconstituting it 1ml saline as detailed in step 3, it's unclear if this heating step should still be carried out? I intend to use small volumes of the 1ml reconsituted adjuvant with 5 different antigens, and then save the rest for immunisations a couple of weeks later. Should the adjuvant be heated initially AND before combining with the relevant antigen? or should it occur just once, either before reconstitution or immediately prior to mixing - if so, which?

Thanks in advance,

Rebecca