I would appreciate if anyone can share information about HA1 immunizations in mice. Particularly Cal09 HA1 in C57BL/6 mice or BALB/c mice. Any information about the murine MHC class2 restriction of Cal09 HA1 will be appreciated.
Thank you.
Check past work of Adam Adler. He used TCR transgenic mice recognising a specific HA epitope, which was MHC class 2 restricted. We strudied this as a model antigen in mice as peptide, and expressed in Vaccinia. Not sure, what you are after, but maybe this helps. best regards Uwe
Article Feeding by the tick, Ixodes scapularis, causes CD4+ T cells ...
Evaluation of Oral Immunization with Recombinant Avian Influenza Virus HA1 Displayed on the Lactococcus lactis Surface and Combined with the Mucosal Adjuvant Cholera Toxin Subunit B ▿
Han Lei,1 Zhina Sheng,2 Qian Ding,2 Jian Chen,2 Xiaohui Wei,2 Dominic Man-Kit Lam,3 and Yuhong Xu2,*
ABSTRACT
The development of safe and efficient avian influenza vaccines for human and animal uses is essential for preventing virulent outbreaks and pandemics worldwide. In this study, we constructed a recombinant (pgsA-HA1 gene fusion) Lactococcus lactis strain that expresses and displays the avian influenza virus HA1 antigens on its surface. The vectors were administered by oral delivery with or without the addition of cholera toxin subunit B (CTB). The resulting immune responses were analyzed, and the mice were eventually challenged with lethal doses of H5N1 viruses. Significant titers of hemagglutinin (HA)-specific serum IgG and fecal IgA were detected in the group that also received CTB. Cellular immunities were also shown in both cell proliferation and gamma interferon (IFN-γ) enzyme-linked immunospot (ELISpot) assays. Most importantly, the mice that received the L. lactis pgsA-HA1 strain combined with CTB were completely protected from lethal challenge of the H5N1 virus. These findings support the further development of L. lactis-based avian influenza virus vaccines for human and animal uses.
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3147322/
Thank you so much for the suggestions. I'm doing protein immunizations with Cal04/09 H1N1 HA1 protein in C57BL/6 mice. It is a primary immunization and the reponse I get with my specific formulation is very weak. I was unable to find any literature evidence that protein immunizations with this specific HA1 protein were performed in wild type mice before(specifically C57BL/6 or BALB/c mice).
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