Immunization

How can I assess the Oral Cholera Vaccine efficacy during a mass vaccination campaign (Sub Saharan Africa)?

I would like to know what is the best approach between prevented number of cases for every 1000 vaccinated individuals and the decreasing lethality of cholera in the vaccinated region when it...

11 March 2015 6,309 4 View

How can I investigate vaccine failures?

We are seeing plenty of cases of vaccine failure as outbreaks of velogenic Newcastle disease and Infectious Bronchitis which are reported in in vaccinated flocks. How do you think should one...

06 March 2015 8,990 9 View

Does anyone have experience in immunization of balb-c for hybbridma and monoclonal antibody production?

I want to know, in troubleshooting in the balb c immunization specially, what the difference between prebleeding and immunized mouse is?

02 March 2015 1,290 9 View

Is Tolerance possible in DNA vaccination?

I'm working on polyclonal antibody production through genetic immunization in rabbits and after 5 month of immunization I got nothing! I need to know is there a problem with Tolerance induction...

02 January 2015 1,894 4 View

Why antibody-mediated immunity to Shigella dysentery requires several episodes of infection to get primed?

Why antibody-mediated immunity to Shigella dysentery requires several episodes of infection to get primed?

07 November 2014 3,162 1 View

How does family structure and religiosity affect child immunization?

By family structure I mean nuclear and single parent family. Also by religiously I mean religious participation and the religious office. 

08 August 2014 6,687 4 View

Prophylactic settings (before a tumour challenge) of anticancer vaccines study. What effects of vaccine application do they reveal?

We are working on a xenogeneous anticancer vaccine (a protein extract of avian-origin cells). Once we performed an experiment in prophylactic settings: there were three vaccinations (one per...

06 June 2014 1,713 3 View

Do children of doctors receive sub-par treatment compared to regular patients?

An interesting situation one commonly encounters. While conversing with many physicians, it inadvertently slips from their ends that they often fall behind on immunizing their children, and even...

17 December 2013 5,697 10 View

How to prepare OVA in CFA?

I need to immunize mice subcutaneously with 500 ug OVA in Complete Freund's Adjuvant. How should I prepare the mix to do it correctly?

10 December 2013 8,314 1 View

Dose of immunization after conjugation of protein to mAb?

I conjugated my 25 KD protein to Anti DEC-205 mAb by polysaccharide groups in Ab structure. I didn't have SMCC or Sulfo-SMCC then I used this protocol for conjugation. Now I have smears with high...

17 November 2013 6,968 1 View

Does anyone have need for a potent but non-reactogenic adjuvant for veterinary or human use?

Our research group have developed a novel polysaccharide adjuvant we call Advax which has proved effective in multiple animal models and is also in late stage human development. Its major virtue...

22 March 2013 8,312 9 View

Do you known about some T cell epitope techniques?

I immunized mice with a recombinant protein and I would like to characterize them against which T cell epitopes the immune response is directed. Which techniques could serve me with this purpose?

28 February 2013 9,374 2 View

What indicators can we use to measure parents loss of confidence in child immunization services?

Presenty, people link hesitance to child immunization with the distance of the health center, availability of vaccines, the child sickness, busy mothers, health workers lack of interpersonal...

25 January 2013 9,261 2 View

How many injectable vaccine can be given at a time? Can a 2 months old child be given more than 2 injections at a time?

Too many vaccines added to the routine vaccination schidule which might require giving two months old child up to 4 injections even with the combined vaccine injection.

21 January 2013 4,426 3 View

Is it worthy to have mass influenza immunization?

Recent CDC report estimates the effectiviness of influenza vaccine by 62%. And we are douting this percent in our area, as most of our patients who got the vaccine reported to have several...

21 January 2013 5,056 3 View

Easy method to remove urea after denaturing purification, no dialysis?

the protein sample will be used for immunization of mice or rabbits, therefore no urea or other chaotrophic chemicals should be present. Precipitation is a benefit and used for concentration of...

07 December 2012 10,060 29 View

Parental attitudinal influence on pediatric influenza immunization

I am doing a research study on the impact of parental attitudinal barriers on Infleunza immunization in the pediactric population. Does anyone have or know of a tool I can convert into survey...

02 December 2012 601 2 View

Antigen specific T cells home to draining lymph nodes after s.c. immunization. How long will it take, before they can be found again in the circulation?

Our immunization schedule is: 3 s.c. shots of antigen on Alum (d0, d14, d28) I would like to know if anybody has tested the re-appearance of memory T cells in blood in a time course experiment....

29 October 2012 550 3 View

Protocol for immunization and treatment of infection in mice

I have a 13 amino acids peptide and I want to inject it in mice to induce immunization. The injection will be in the tail vein. After 12 days I expect to have a peak in the production of...

01 January 1970 4,472 4 View

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