I have tried extraction of RNA from various commercial rabies vaccine which currently used for pre exposure and post exposure for dogs. After that I have done RT- PCR, while running gel i got band for only one vaccine, i tried several times with several type of procedures, all the times i got band from only one vaccine. what may be reasons? is it due to some inhibitor substances which present in vaccines? simultaneously i have tested the serum samples of dog after the immunization for sero- protection assessment, dogs vaccinated with that vaccine showed band in RT PCR had better sero- protection compared to that of other vaccine. Is there any relation between this two?
I am not sure which vaccines you tried, but these days most of the Rabies vaccines are recombinant or synthetic proteins. Therefore you should not expect any RNA or DNA in these vaccines. The old Sheep brain (attenuated) vaccine is no more in use.
I think it depends on the particular vaccines and dr singh well described that point.
Dear Bharathy, you have not stated the purpose of your research however I would say that most of the vaccine are G protein or other proteins fragments and thus RNA can not be extracted.
May be a few ( Say Vaxirab-N) vaccines that use whole virion as component can be helpful in getting the RNA.
You have used the rabies vaccine that is of only one sero-type, so you should not be surprised of the answer you got. The degree of the sero protection obtained showed the quality of the immunogenicity of the vaccine to have evoked that quality of response obtained.
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