I am working on mouse brain coronal section. I am doing a quantification of activated cells in several areas by cFos staining. I am having a hard time doing it on the auditory cortex. I wonder If someone knows any auditory cortex marker for a double staining that I might use in a double staining or any other way?
Thank you for your time!! :)
i don't have the answer, just some tracks. Perhaps you should look at the works of Zilles' team in humans, like a staining some receptors or on myelin (weigert?) could work (primary auditory cortex is highly myelinated). From Moerel et al. (2014) : "Zilles et al. (2002) mapped the human cortex based on multiple transmitter receptors (Zilles et al., 2002; Morosan et al., 2005). They found that human PAC contained a high density of cholinergic muscarinic M2 and nicotinic receptions, most densely expressed in middle cortical layers. Both M2 and nicotinic receptor density sharply dropped at the lateral border of PAC with the belt (Clarke and Morosan, 2012)."
Another track would be to look at studies working on the organization of auditory cortical fields in rodents, they should use some staining of interest for you.
- Badges
- Science topic
- Similar topics
- Methodology
- Laboratory
- Histological Techniques