How do you count DAPI stained cells other than hand counting?

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Sönke Weinert

That should solve your problem:

sorry the link was missing - I worte mobil. I would also recomment ImageJ:

here the original tutorial link:

http://imagejdocu.tudor.lu/doku.php?id=video:analysis:particle_counting_-_automated_and_manual

Best Soenke

Eliseo A Eugenin

several programs, including free ones allow you to count by circularity and intensity. Include your parameters (size and circularity) and the programs are mostly automatic for that

Konstantin Davide Bergmeister

ImageJ is an open access platform with several macros that allow automatic quantification. Google: imageJ dapi quantifaction.

That should help!