Vitamin E, being strongly hydrophobic, tends to not dissolve in PBS/ PBS + tween 80/ethanol. Is there any way to solubilize vitamin E in PBS while preparing a standard curve for drug release?
And, can methanol be used as a release medium?
Use a small volume of a miscible solvent like ethanol, methanol, or DMSO to completely dissolve your vitamin E. Then, slowly dilute it with PBS to reach the desired final concentration. Avoid exceeding the critical micelle concentration (CMC) of the surfactant used in PBS, as it can decrease solubility.
Either you can use heating and sonication method for this solubility purpose.
Thank you for the suggestion Rahul Pal .
Would you please mention why exceeding the CMC can decrease solubility? As exceeding the CMC of surfactants usually increases the solubility of hydrophobic drugs.
Yes because if At high surfactant concentrations above the CMC, micelles can grow in size and become less densely packed. This can reduce the surface area available for solubilizing hydrophobic molecules, leading to decreased drug solubility.
Large, weakly-associated aggregates may be less effective in solubilizing the drug compared to smaller, tightly packed micelles formed near the CMC.
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